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Literature summary for 2.5.1.93 extracted from
- Functional characterization of LePGT1, a membrane-bound prenyltransferase involved in the geranylation of p-hydroxybenzoic acid (2009), Biochem. J., 421, 231-241.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| all point mutants and chimeric enzymes are constitutively expressed in Saccharomyces cerevisiae containing a disrupted copy of the COQ2 gene | Lithospermum erythrorhizon |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D208A | no activity | Lithospermum erythrorhizon |
| D211A | enzyme activity is 1.12% of wild-type activity | Lithospermum erythrorhizon |
| D212A | enzyme activity is 0.11% of wild-type activity | Lithospermum erythrorhizon |
| D84A | enzyme activity is 1.28% of wild-type activity | Lithospermum erythrorhizon |
| D87A | no activity | Lithospermum erythrorhizon |
| D91A | no activity | Lithospermum erythrorhizon |
| K152A | enzyme activity is 0.15% of wild-type activity | Lithospermum erythrorhizon |
| K229A | enzyme activity is 12.9% of wild-type activity | Lithospermum erythrorhizon |
| additional information | the amino acid residues of LePGT1 critical for the enzymatic activity and the region responsible for the binding of the substrates are elucidated by mutational analysis. Substrate specificity is analysed using chimeric enzymes derived from LePGT1 and UbiA (EC 2.5.1.39). In vitro and in vivo analysis of the chimeras suggests that the determinant region for this specificity is within 130 amino acids of the N-terminal | Lithospermum erythrorhizon |
| N83A | enzyme activity is 0.82% of wild-type activity | Lithospermum erythrorhizon |
| Q207A | enzyme activity is 2.31% of wild-type activity | Lithospermum erythrorhizon |
| R153A | enzyme activity is 81.6% of wild-type activity | Lithospermum erythrorhizon |
| R76A | enzyme activity is 0.26% of wild-type activity | Lithospermum erythrorhizon |
| R96A | enzyme activity is 0.22% of wild-type activity | Lithospermum erythrorhizon |
| S219A | enzyme activity is 14.7% of wild-type activity | Lithospermum erythrorhizon |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0022 | - |
geranyl diphosphate | pH 7.6, 30°C, mutant enzyme D208E | Lithospermum erythrorhizon |  |
| 0.0213 | - |
4-hydroxybenzoate | pH 7.6, 30°C, mutant enzyme D212E | Lithospermum erythrorhizon | |
| 0.0295 | - |
geranyl diphosphate | pH 7.6, 30°C, wild-type enzyme | Lithospermum erythrorhizon | |
| 0.0305 | - |
4-hydroxybenzoate | pH 7.6, 30°C, mutant enzyme D208E | Lithospermum erythrorhizon | |
| 0.0365 | - |
geranyl diphosphate | pH 7.6, 30°C, mutant enzyme D212E | Lithospermum erythrorhizon | |
| 0.0664 | - |
4-hydroxybenzoate | pH 7.6, 30°C, wild-type enzyme | Lithospermum erythrorhizon | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Lithospermum erythrorhizon | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranyl diphosphate + 4-hydroxybenzoate | Lithospermum erythrorhizon | regulatory enzyme for the biosynthesis of shikonin, a naphthoquinone pigment | 3-geranyl-4-hydroxybenzoate + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lithospermum erythrorhizon | Q8W405 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranyl diphosphate + 4-hydroxybenzoate | regulatory enzyme for the biosynthesis of shikonin, a naphthoquinone pigment | Lithospermum erythrorhizon | 3-geranyl-4-hydroxybenzoate + diphosphate | - |
? | |
| geranyl diphosphate + 4-hydroxybenzoate | LePGT1 can utilize only geranyl diphosphate as its prenyl substrate | Lithospermum erythrorhizon | 3-geranyl-4-hydroxybenzoate + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LePGT1 | - |
Lithospermum erythrorhizon |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Lithospermum erythrorhizon |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Lithospermum erythrorhizon |
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