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Literature summary for 2.5.1.98 extracted from

  • Ivashina, T.V.; Fedorova, E.E.; Ashina, N.P.; Kalinchuk, N.A.; Druzhinina, T.N.; Shashkov, A.S.; Shibaev, V.N.; Ksenzenko, V.N.
    Mutation in the pssM gene encoding ketal pyruvate transferase leads to disruption of Rhizobium leguminosarum bv. viciae-Pisum sativum symbiosis (2010), J. Appl. Microbiol., 109, 731-742.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + [D-GlcA-beta-(1->4)-2-O-Ac-D-GlcA-beta-(1->4)-D-Glc-beta-(1->4)-[3-O-CH3-CH2CH(OH)C(O)-D-Gal-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->6)]-2(or3)-O-Ac-D-Glc-alpha-(1->6)]n Rhizobium leguminosarum
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[D-GlcA-beta-(1->4)-2-O-Ac-D-GlcA-beta-(1->4)-D-Glc-beta-(1->4)-[3-O-CH3-CH2CH(OH)C(O)-D-Gal-beta-(1->3)-4,6-CH3(COO-)C-D-Glc-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->6)]-2(or3)-O-Ac-D-Glc-alpha-(1->6)]n + phosphate
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Organism

Organism UniProt Comment Textmining
Rhizobium leguminosarum Q9FCP2
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + [D-GlcA-beta-(1->4)-2-O-Ac-D-GlcA-beta-(1->4)-D-Glc-beta-(1->4)-[3-O-CH3-CH2CH(OH)C(O)-D-Gal-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->6)]-2(or3)-O-Ac-D-Glc-alpha-(1->6)]n
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Rhizobium leguminosarum [D-GlcA-beta-(1->4)-2-O-Ac-D-GlcA-beta-(1->4)-D-Glc-beta-(1->4)-[3-O-CH3-CH2CH(OH)C(O)-D-Gal-beta-(1->3)-4,6-CH3(COO-)C-D-Glc-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->6)]-2(or3)-O-Ac-D-Glc-alpha-(1->6)]n + phosphate
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General Information

General Information Comment Organism
malfunction knock-out of pssM leads to the absence of the pyruvic acid ketal group at the subterminal glucose in the repeating unit of exopolysaccharide (shown by 13C and 1H nuclear magnetic resonance analysis) Rhizobium leguminosarum
physiological function Rhizobium leguminosarum bv. viciae strain VF39 synthesizes an acidic exopolysaccharide, which is necessary to establish nitrogen-fixing symbiosis with Pisum sativum, Vicia faba, and Vicia sativa. This exopolysaccharide is a polymer of an octasaccharide repeating unit consisting of a backbone of two glucose and two glucuronic acid residues, a side chain of three glucose and one gaThe pssM gene encodes ketal pyruvate transferase, which is responsible for pyruvylation of subterminal glucose in the EPS repeating unit Rhizobium leguminosarum