Crystallization (Comment) | Organism |
---|---|
concentrated isoenzyme AAT-1 solution, 10 mM Tris-HCl, pH 8.0, 1 mM 2-oxoglutarate, 2 mM KOH, 0.01 pyridoxal 5'-phosphate, 0.01% 2-mercaptoethanol, polyethylene glycol 6000 8% w/v, 4°C, 6 days | Oryza sativa |
concentrated isoenzyme AAT-2 solution, 10 mM potassium phosphate, pH 7.0, 1 mM 2-oxoglutarate, 2 mM KOH, 0.001 mM pyridoxal 5'-phosphate, 0.01% 2-mercaptoethanol, dialysis against distilled water at 4°C for 3 days | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
oxaloacetate | isoenzyme AAT-1 | Oryza sativa | |
0.048 | - |
oxaloacetate | isoenzyme AAT-2 | Oryza sativa | |
0.105 | - |
2-oxoglutarate | isoenzyme AAT-2, pH 7.8, 37°C | Oryza sativa | |
0.11 | - |
2-oxoglutarate | isoenzyme AAT-1, pH 7.8, 37°C | Oryza sativa | |
2.4 | - |
L-aspartate | isoenzyme AAT-1, pH 7.8, 37°C | Oryza sativa | |
3.7 | - |
L-aspartate | isoenzyme AAT-2, pH 7.8, 37°C | Oryza sativa | |
5 | - |
L-tryptophan | L-glutamate, isoenzyme AAT-1 | Oryza sativa | |
13.2 | - |
L-glutamate | isoenzyme AAT-2 | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
2 * 44000, isozyme AAT-1, SDS-PAGE | Oryza sativa |
47000 | - |
2 * 47000, AAT-2, SDS-PAGE | Oryza sativa |
88000 | - |
gel filtration | Oryza sativa |
88000 | - |
isozyme AAT-1 | Oryza sativa |
94000 | - |
isozyme AAT-2, gel filtration | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | Oryza sativa | - |
oxaloacetate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
cv. Koganemasari | - |
Oryza sativa | - |
2 isoenzymes: AAT-1, AAT-2 | - |
Purification (Comment) | Organism |
---|---|
2 isoenzymes: AAT-1, AAT-2 | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Oryza sativa | - |
root | - |
Oryza sativa | - |
seed coat | bran | Oryza sativa | - |
stem | - |
Oryza sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Oryza sativa |
390 | - |
purified isozyme AAT-1 | Oryza sativa |
450 | - |
purified isozyme AAT-2 | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Oryza sativa | oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | - |
Oryza sativa | oxaloacetate + L-glutamate | - |
r | |
L-cysteine sulfinic acid + 2-oxoglutarate | 63% of the activity with aspartate | Oryza sativa | 2-oxo-3-sulfinopropionic acid + L-glutamate | - |
? | |
L-glutamate + 2-oxoglutarate | L-glutamate is best amino acid donor | Oryza sativa | 2-oxoglutarate + L-glutamate | - |
? | |
L-phenylalanine + 2-oxoglutarate | - |
Oryza sativa | 2-oxo-3-phenylpropionic acid + L-glutamate | i.e. phenylpyruvate | ? | |
L-tryptophan + 2-oxoglutarate | - |
Oryza sativa | 3-indole-2-oxopropionic acid + L-glutamate | - |
? | |
additional information | substrate specificity | Oryza sativa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 44000, isozyme AAT-1, SDS-PAGE | Oryza sativa |
dimer | 2 * 47000, AAT-2, SDS-PAGE | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | forward reaction, isoenzymes AAT-1 and AAT-2 | Oryza sativa |
8 | 8.5 | reverse reaction, isoenzyme AAT-1 | Oryza sativa |
8 | 8.8 | reverse reaction, isoenzyme AAT-2 | Oryza sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | a pyridoxal 5'-phosphate protein | Oryza sativa | |
pyridoxal 5'-phosphate | isozyme AAT-1: 0.87 mol per mol of subunit, isozyme AAT-2: 0.93 mol per mol of subunit | Oryza sativa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Oryza sativa | isozyme AAT-2 | - |
5 |
Oryza sativa | isozyme AAT-1 | - |
6.5 |