Cloned (Comment) | Organism |
---|---|
overexpression from plasmid in strain JM103 | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
sitting drop method, protein solution: 30 mg/ml, 10 mM potassium phosphate, pH 7.0, 0.01 mM pyridoxal 5'-phosphate, 1 mM EDTA, 2 mM succinate, 0.3 mM azide, reservoir solution: same composition as protein solution, contains no protein but polyethylene glycol 4000, 11-17% w/v, 20°C, stepwise addition of protein solution, X-ray diffraction analysis | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | Escherichia coli | - |
oxaloacetate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinantly overexpressed enzyme | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
232 | - |
purified recombinant enyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Escherichia coli | oxaloacetate + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000-45000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Escherichia coli |