Cloned (Comment) | Organism |
---|---|
cloning and DNA-sequencing of cDNA encoding the plastidic isozyme pAspAT, DNA and amino acid sequence analysis | Panicum miliaceum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
oxaloacetate | isozyme pAspAT, gamma-form, pH 8.0, 25°C | Panicum miliaceum | |
0.03 | - |
oxaloacetate | isozyme pAspAT, alpha-form, pH 8.0, 25°C | Panicum miliaceum | |
0.033 | - |
oxaloacetate | isozyme pAspAT, beta-form, pH 8.0, 25°C | Panicum miliaceum | |
0.056 | - |
oxaloacetate | isozyme mAspAT, pH 8.0, 25°C | Panicum miliaceum | |
0.065 | - |
oxaloacetate | isozyme cAspAT, alpha-form, pH 8.0, 25°C | Panicum miliaceum | |
0.07 | - |
2-oxoglutarate | isozyme pAspAT, gamma-form, pH 8.0, 25°C | Panicum miliaceum | |
0.075 | - |
2-oxoglutarate | isozyme pAspAT, beta-form, pH 8.0, 25°C | Panicum miliaceum | |
0.08 | - |
oxaloacetate | isozyme cAspAT, gamma-form, pH 8.0, 25°C | Panicum miliaceum | |
0.085 | - |
oxaloacetate | isozyme cAspAT, beta-form, pH 8.0, 25°C | Panicum miliaceum | |
0.087 | - |
2-oxoglutarate | isozyme pAspAT, alpha-form, pH 8.0, 25°C | Panicum miliaceum | |
0.13 | - |
2-oxoglutarate | isozyme mAspAT, pH 8.0, 25°C | Panicum miliaceum | |
0.14 | - |
2-oxoglutarate | isozyme cAspAT, beta-form, pH 8.0, 25°C | Panicum miliaceum | |
0.17 | - |
2-oxoglutarate | isozyme cAspAT, alpha-form, pH 8.0, 25°C | Panicum miliaceum | |
0.2 | - |
2-oxoglutarate | isozyme cAspAT, gamma-form, pH 8.0, 25°C | Panicum miliaceum | |
1.3 | - |
L-aspartate | isozyme mAspAT, pH 8.0, 25°C | Panicum miliaceum | |
2.2 | - |
L-aspartate | isozyme pAsp-AT, beta-form, pH 8.0, 25°C | Panicum miliaceum | |
2.3 | - |
L-aspartate | isozyme pAsp-AT, gamma-form, pH 8.0, 25°C | Panicum miliaceum | |
2.6 | - |
L-aspartate | isozyme pAsp-AT and cAspAT, alpha-form, pH 8.0, 25°C | Panicum miliaceum | |
3 | - |
L-aspartate | isozyme cAspAT, gamma-form, pH 8.0, 25°C | Panicum miliaceum | |
3.2 | - |
L-aspartate | isozyme cAspAT, beta-form, pH 8.0, 25°C | Panicum miliaceum | |
7.5 | - |
L-glutamate | isozyme mAspAT, pH 8.0, 25°C | Panicum miliaceum | |
13 | - |
L-glutamate | isozyme cAspAT, gamma-form, pH 8.0, 25°C | Panicum miliaceum | |
14 | - |
L-glutamate | isozyme cAspAT, beta-form, pH 8.0, 25°C | Panicum miliaceum | |
17 | - |
L-glutamate | isozyme cAspAT, alpha-form, pH 8.0, 25°C | Panicum miliaceum | |
18 | - |
L-glutamate | isozyme pAspAT, gamma-form, pH 8.0, 25°C | Panicum miliaceum | |
20 | - |
L-glutamate | isozyme pAspAT, beta-form, pH 8.0, 25°C | Panicum miliaceum | |
32 | - |
L-glutamate | isozyme pAspAT, alpha-form, pH 8.0, 25°C | Panicum miliaceum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | isozyme pAspAT, multiple forms | Panicum miliaceum | 9507 | - |
cytosol | multiple catalytically active forms of the cytosolic enzyme | Panicum miliaceum | 5829 | - |
mitochondrion | multiple catalytically active forms of mitochondrial isozyme | Panicum miliaceum | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | Panicum miliaceum | - |
oxaloacetate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Panicum miliaceum | - |
at least 3 isozymes: inducible cytosolic cAspAT and mitochondrial mAspAT, and a constitutive minor plastidic pAspAT | - |
Purification (Comment) | Organism |
---|---|
isozymes mAsp-AT, cAsp-AT, and pAspAT from young green leaves | Panicum miliaceum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | green, greening and etiolated: all isozymes, low content in etiolated leaves | Panicum miliaceum | - |
mesocotyl | cytosolic and mitochondrial isozymes, low content | Panicum miliaceum | - |
root | cytosolic isozyme, low content | Panicum miliaceum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
178 | - |
purified pAspAT, gamma-form | Panicum miliaceum |
252 | - |
purified pAspAT, alpha-form | Panicum miliaceum |
322 | - |
purified pAspAT, beta-form | Panicum miliaceum |
512 | - |
purified mAspAT | Panicum miliaceum |
671 | - |
purified cAspAT, alpha-form | Panicum miliaceum |
953 | - |
purified cAspAT, gamma-form | Panicum miliaceum |
1035 | - |
purified cAspAT, beta-form | Panicum miliaceum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Panicum miliaceum | oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | - |
Panicum miliaceum | oxaloacetate + L-glutamate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
AspAT | - |
Panicum miliaceum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Panicum miliaceum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Panicum miliaceum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Panicum miliaceum | multiple forms of isozyme cAspAT and pAspAT have different isolelectric points, pI mAspAT: pH 4.93, pI cAspATs: pH 5.54-5.76, pI pAspATs: pH 4.94-5.13 | - |
additional information |