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Literature summary for 2.6.1.1 extracted from

  • Mohamed, T.M.
    Purification and characterization of aspartate aminotransferase from developing embryos of the camel tick Hyalomma dromedarii (2001), Exp. Appl. Acarol., 25, 231-244.
    View publication on PubMed
Search for more literature for 2.6.1.1

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ AST II Hyalomma dromedarii
Mg2+ AST II Hyalomma dromedarii
Ni2+ AST II Hyalomma dromedarii
Zn2+ AST II Hyalomma dromedarii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
 2-oxoglutarate isozyme AST II, 37°C, pH 7.5 Hyalomma dromedarii
15.1
-
 L-aspartate isozyme AST II, 37°C, pH 7.5 Hyalomma dromedarii

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activation, isozyme AST II Hyalomma dromedarii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 1 * 50000, SDS-PAGE Hyalomma dromedarii
52000
-
 isozyme AST II, gel filtration Hyalomma dromedarii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Hyalomma dromedarii
-
 oxaloacetate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Hyalomma dromedarii
-
 camel tick
-
Hyalomma dromedarii
-
 4 isozymes: AST I , AST II, AST III, AST IV
-

Purification (Commentary)

Purification (Comment) Organism
isozyme AST II, 30.25fold Hyalomma dromedarii

Source Tissue

Source Tissue Comment Organism Textmining
zygote 3-days-old eggs Hyalomma dromedarii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.061
-
 partially purified isozyme AST II Hyalomma dromedarii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
 Hyalomma dromedarii oxaloacetate + L-glutamate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE Hyalomma dromedarii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Hyalomma dromedarii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 isozyme AST II, 15 min, stable Hyalomma dromedarii
80
-
 isozyme AST II, loss of 89% activity Hyalomma dromedarii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 isozyme AST II Hyalomma dromedarii