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Literature summary for 2.6.1.1 extracted from
- Regulation of 2-oxoglutarate metabolism in rat liver by NADP-isocitrate dehydrogenase and aspartate aminotransferase (2006), Biochemistry, 71, 211-217.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| aconitate | activating effect on the activity of the cytoplasmic enzyme form | Rattus norvegicus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fumarate | - |
Rattus norvegicus | |
| isocitrate | - |
Rattus norvegicus | |
| L-aspartate | - |
Rattus norvegicus | |
| L-glutamate | - |
Rattus norvegicus | |
| L-glutamine | - |
Rattus norvegicus | |
| succinate | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
L-aspartate | cytoplasmic enzyme | Rattus norvegicus | |
| 0.75 | - |
L-aspartate | mitochondrial enzyme | Rattus norvegicus | |
| 1.25 | - |
2-oxoglutarate | cytoplasmic enzyme | Rattus norvegicus | |
| 1.75 | - |
2-oxoglutarate | mitochondrial enzyme | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Rattus norvegicus | 5737 | - |
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 92000 | - |
mitochondrial form, SDS-PAGE | Rattus norvegicus |
| 98000 | - |
cytoplasmic form, SDS-PAGE | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| differential centrifugation, Sephadex G-25 gel filtration, DEAE cellulose ion exchange chromatography, and Sephadex G-150 gel filtration | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 22.37 | - |
mitochondrial enzyme | Rattus norvegicus |
| 48.45 | - |
cytoplasmic enzyme | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-aspartate + 2-oxoglutarate | - |
Rattus norvegicus | oxaloacetate + L-glutamate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AsAT | - |
Rattus norvegicus |
| aspartate aminotransferase | - |
Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
mitochondrial form | Rattus norvegicus |
| 7.5 | - |
cytoplasmic form | Rattus norvegicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
L-aspartate | cytoplasmic enzyme | Rattus norvegicus | |
| 1.8 | - |
L-glutamate | mitochondrial enzyme | Rattus norvegicus | |
| 1.9 | - |
L-aspartate | mitochondrial enzyme | Rattus norvegicus | |
| 2.4 | - |
L-glutamate | cytoplasmic enzyme | Rattus norvegicus | |
| 2.6 | - |
L-glutamine | mitochondrial enzyme | Rattus norvegicus | |
| 3.7 | - |
L-glutamine | cytoplasmic enzyme | Rattus norvegicus | |
| 4.1 | - |
succinate | mitochondrial enzyme | Rattus norvegicus | |
| 5.7 | - |
succinate | cytoplasmic enzyme | Rattus norvegicus | |
| 6.6 | - |
isocitrate | cytoplasmic enzyme | Rattus norvegicus | |
| 8.4 | - |
fumarate | cytoplasmic enzyme | Rattus norvegicus | |
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