Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
2-oxoglutarate | pH 8.5, 60°C, recombinant enzyme | Saccharolobus solfataricus | |
2.05 | - |
L-cysteine sulfinic acid | pH 8.5, 60°C, recombinant enzyme | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 53000, SDS-PAGE | Saccharolobus solfataricus |
120000 | - |
gel filtration | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus MT-4 / DSM 5833 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
93 | - |
pH 8.5, 60°C, recombinant enzyme | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine sulfinic acid + 2-oxoglutarate | - |
Saccharolobus solfataricus | 2-oxo-3-sulfinopropionic acid + L-glutamate | - |
? | |
L-cysteine sulfinic acid + 2-oxoglutarate | - |
Saccharolobus solfataricus MT-4 / DSM 5833 | 2-oxo-3-sulfinopropionic acid + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, SDS-PAGE | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
AspATSs | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
enzymes starts to precipitate at temperatures above 100°C | Saccharolobus solfataricus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharolobus solfataricus | isoelectric focusing, pH range 4.0-7.5, recombinant enzyme | - |
6.3 |