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Literature summary for 2.6.1.101 extracted from
- The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233: characterization of an aminotransferase involved in the formation of 2-deoxystreptamine (2005), Org. Biomol. Chem., 3, 1410-1418.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene neo-6, DNA and amino acid sequence determination and analysis, overexpression as His6-tagged enzyme in Esccherichia coli | Streptomyces fradiae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 46951, sequence calculation, x * 45000, recombinant His6-tagged enzyme, SDS-PAGE, x * 46801, recombinant His6-tagged enzyme, mass spectrometry | Streptomyces fradiae |
| 46801 | - |
x * 46951, sequence calculation, x * 45000, recombinant His6-tagged enzyme, SDS-PAGE, x * 46801, recombinant His6-tagged enzyme, mass spectrometry | Streptomyces fradiae |
| 46951 | - |
x * 46951, sequence calculation, x * 45000, recombinant His6-tagged enzyme, SDS-PAGE, x * 46801, recombinant His6-tagged enzyme, mass spectrometry | Streptomyces fradiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose | Streptomyces fradiae | - |
2-oxoglutaramate + 2-deoxystreptamine | - |
? |  |
| L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose | Streptomyces fradiae NCIMB 8233 | - |
2-oxoglutaramate + 2-deoxystreptamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces fradiae | - |
- |
- |
| Streptomyces fradiae | Q53U20 | gene neo-6 encoded in the neo gene cluster | - |
| Streptomyces fradiae NCIMB 8233 | - |
- |
- |
| Streptomyces fradiae NCIMB 8233 | Q53U20 | gene neo-6 encoded in the neo gene cluster | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose | - |
Streptomyces fradiae | 2-oxoglutaramate + 2-deoxystreptamine | - |
? | |
| L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose | - |
Streptomyces fradiae NCIMB 8233 | 2-oxoglutaramate + 2-deoxystreptamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 46951, sequence calculation, x * 45000, recombinant His6-tagged enzyme, SDS-PAGE, x * 46801, recombinant His6-tagged enzyme, mass spectrometry | Streptomyces fradiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| btrR | - |
Streptomyces fradiae |
| BtrS | - |
Streptomyces fradiae |
| L-glutamine: 2-deoxy-scyllo-inosamine aminotransferase | - |
Streptomyces fradiae |
| L-glutamine:DOI aminotransferase | - |
Streptomyces fradiae |
| neo-6 | - |
Streptomyces fradiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Streptomyces fradiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Streptomyces fradiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | the BtrR-bound pyridoxal 5'-phosphate is the essential cofactor for the amino-transfer activity | Streptomyces fradiae | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | BtrR and StsC are the two homologues of Neo-6 in butirosin and streptamycin biosynthetic pathways | Streptomyces fradiae |
| metabolism | purified Neo-6 also catalyses the amino transfer from L-glutamine to 2-deoxyscyllo-inosose to produce 2-deoxyscyllo-inosamine when coupled with BtrC in the presence of G6P, NAD+, Co2+ and L-glutamine, EC 2.6.1.100 | Streptomyces fradiae |
| additional information | gene neo6 of the neo gene cluster and btrR from the btr gene cluster are equivalent, genetic organization, overview | Streptomyces fradiae |
| physiological function | the enzyme produces 2-deoxystreptamine (DOS), which is the aglycone in neomycins, overview. Neo-6 is also the enzyme catalysing the transamination of 2-deoxy-scyllo-inosose, DOI, using L-glutamine as an amino donor to produce 2-deoxyscyllo-inosamine as required for 2-deoxystreptamine biosynthesis, function of Neo-6 as the L-glutamine:DOI aminotransferase | Streptomyces fradiae |
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Release 2023.1 (January 2023)
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