Cloned (Comment) | Organism |
---|---|
gene neoB, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli | Streptomyces fradiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose | Streptomyces fradiae | - |
2-oxoglutaramate + 2-deoxystreptamine | - |
? | |
L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose | Streptomyces fradiae NBRC 12773 | - |
2-oxoglutaramate + 2-deoxystreptamine | - |
? | |
additional information | Streptomyces fradiae | NeoB also shows L-glutamine:2-deoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-scyllo-inosamine, EC 2.6.1.100 | ? | - |
? | |
additional information | Streptomyces fradiae NBRC 12773 | NeoB also shows L-glutamine:2-deoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-scyllo-inosamine, EC 2.6.1.100 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces fradiae | Q53U20 | gene neoB, encoded in the neomycin biosynthetic gene cluster | - |
Streptomyces fradiae NBRC 12773 | Q53U20 | gene neoB, encoded in the neomycin biosynthetic gene cluster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose | - |
Streptomyces fradiae | 2-oxoglutaramate + 2-deoxystreptamine | - |
? | |
L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose | - |
Streptomyces fradiae NBRC 12773 | 2-oxoglutaramate + 2-deoxystreptamine | - |
? | |
additional information | NeoB also shows L-glutamine:2-deoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-scyllo-inosamine, EC 2.6.1.100 | Streptomyces fradiae | ? | - |
? | |
additional information | NeoB also shows L-glutamine:2-deoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-scyllo-inosamine, EC 2.6.1.100 | Streptomyces fradiae NBRC 12773 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Gln:DOI aminotransferase | - |
Streptomyces fradiae |
L-glutamine:DOI aminotransferase | - |
Streptomyces fradiae |
Neo6 | - |
Streptomyces fradiae |
neoB | - |
Streptomyces fradiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Streptomyces fradiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Streptomyces fradiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Streptomyces fradiae |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the biosynthesis of the 2-deoxystreptamine (DOS)-containing aminoglycoside neomycin. 3-Amino-2,3-dideoxy-scyllo-inosose produced by the NeoA reaction is continuously used as substrate for the natural forward transamination catalyzed by NeoB in the 2-deoxystreptamine biosynthesis. NeoB also shows L-glutamine:2-deoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-scyllo-inosamine, EC 2.6.1.100 | Streptomyces fradiae |
additional information | the equivalent of gene neoB, from neo gene cluster, in the btr gene cluster is gene btrS | Streptomyces fradiae |