Literature summary for 2.6.1.105 extracted from

Van Arsdell, S.W.; Perkins, J.B.; Yocum, R.R.; Luan, L.; Howitt, C.L.; Chatterjee, N.P.; Pero, J.G.
Removing a bottleneck in the Bacillus subtilis biotin pathway: bioA utilizes lysine rather than S-adenosylmethionine as the amino donor in the KAPA-to-DAPA reaction (2005), Biotechnol. Bioeng., 91, 75-83.

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Inhibitors

Inhibitors	Comment	Organism	Structure
8-Amino-7-oxononanoate	-	Bacillus subtilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2	-	L-lysine	pH 8.3, 37°C	Bacillus subtilis
25	-	8-Amino-7-oxononanoate	pH 8.3, 37°C	Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-lysine + 8-amino-7-oxononanoate	Bacillus subtilis	the enzyme participates in the pathway for biotin biosynthesis	(S)-2-amino-6-oxohexanoate + 7,8-diaminononanoate	(S)-2-amino-6-oxohexanoate i.e. L-2-aminoadipate 6-semialdehyde i.e. L-allysine	?
L-lysine + 8-amino-7-oxononanoate	Bacillus subtilis 168	the enzyme participates in the pathway for biotin biosynthesis	(S)-2-amino-6-oxohexanoate + 7,8-diaminononanoate	(S)-2-amino-6-oxohexanoate i.e. L-2-aminoadipate 6-semialdehyde i.e. L-allysine	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	P53555	-	-
Bacillus subtilis 168	P53555	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-lysine + 8-amino-7-oxononanoate	the enzyme participates in the pathway for biotin biosynthesis	Bacillus subtilis	(S)-2-amino-6-oxohexanoate + 7,8-diaminononanoate	(S)-2-amino-6-oxohexanoate i.e. L-2-aminoadipate 6-semialdehyde i.e. L-allysine	?
L-lysine + 8-amino-7-oxononanoate	higly specific for L-lysine as amino donor	Bacillus subtilis	(S)-2-amino-6-oxohexanoate + 7,8-diaminononanoate	(S)-2-amino-6-oxohexanoate i.e. L-2-aminoadipate 6-semialdehyde i.e. L-allysine	?
L-lysine + 8-amino-7-oxononanoate	the enzyme participates in the pathway for biotin biosynthesis	Bacillus subtilis 168	(S)-2-amino-6-oxohexanoate + 7,8-diaminononanoate	(S)-2-amino-6-oxohexanoate i.e. L-2-aminoadipate 6-semialdehyde i.e. L-allysine	?
L-lysine + 8-amino-7-oxononanoate	higly specific for L-lysine as amino donor	Bacillus subtilis 168	(S)-2-amino-6-oxohexanoate + 7,8-diaminononanoate	(S)-2-amino-6-oxohexanoate i.e. L-2-aminoadipate 6-semialdehyde i.e. L-allysine	?

Synonyms

Synonyms	Comment	Organism
bioA	gene name, ambiguous	Bacillus subtilis
bioK	-	Bacillus subtilis
DAPA aminotransferase	ambiguous	Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Bacillus subtilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.3	-	assay at	Bacillus subtilis

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Bacillus subtilis

General Information

General Information	Comment	Organism
physiological function	the enzyme participates in the pathway for biotin biosynthesis	Bacillus subtilis

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Release 2023.1 (January 2023)