Literature summary for 2.6.1.13 extracted from

Rosenthal, G.A.; Dahlman, D.L.
Interaction of L-canaline with ornithine aminotransferase of the tobacco hornworm, Manduca sexta (Sphingidae) (1990), J. Biol. Chem., 265, 868-873.

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General Stability

General Stability	Organism
2-mercaptoethanol or DTT stabilizes	Manduca sexta

Inhibitors

Inhibitors	Comment	Organism	Structure
L-canaline	binds covalently to enzyme forming a canaline-pyridoxal phosphate oxime, dialysis against free pyridoxal phosphate slowly reactivates the enzyme as the oxime is replaced by pyridoxal phosphate	Manduca sexta
N-ethylmaleimide	complete inactivation at 0.1 and 1 mM after 20 min	Manduca sexta

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.3	-	L-ornithine	pH 8, 37°C	Manduca sexta
3.2	-	2-oxoglutarate	pH 8, 37°C	Manduca sexta

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
36000	-	4 * 36000, SDS-PAGE	Manduca sexta
148000	152000	nondenaturing PAGE, gel filtration	Manduca sexta

Organism

Organism	UniProt	Comment	Textmining
Manduca sexta	-	tobacco hornworm	-

Purification (Commentary)

Purification (Comment)	Organism
-	Manduca sexta

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.6	-	-	Manduca sexta

Storage Stability

Storage Stability	Organism
-60°C, long-term storage is best as a pellet under liquid-saturated ammonium sulfate	Manduca sexta
1°C, sharp loss in activity, especially in absence of free pyridoxal phosphate and mercaptans	Manduca sexta

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-ornithine + 2-oxoglutarate	-	Manduca sexta	DELTA1-pyrroline-5-carboxylate + L-glutamate	DL-pyrroline-5-carboxylate	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 36000, SDS-PAGE	Manduca sexta

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Manduca sexta

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0333	-	2-oxoglutarate	-	Manduca sexta
0.0333	-	L-ornithine	-	Manduca sexta

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	a pyridoxal phosphate protein	Manduca sexta
pyridoxal 5'-phosphate	4 mol of pyridoxal phosphate per mol of enzyme	Manduca sexta

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Release 2023.1 (January 2023)