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Literature summary for 2.6.1.13 extracted from
- Biochemical characterization, homology modeling and docking studies of ornithine delta-aminotransferase - an important enzyme in proline biosynthesis of plants (2007), J. Mol. Graph. Model., 26, 709-719.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | omission from the reaction mixture reduces the activity by 60% | Vigna aconitifolia |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Vigna aconitifolia |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| homology modeling of crystal structure and flexible docking studies. G106 and K256 are the important residues in binding | Vigna aconitifolia |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-isoleucine | - |
Vigna aconitifolia | |
| L-serine | - |
Vigna aconitifolia | |
| L-valine | - |
Vigna aconitifolia | |
| additional information | not inhibitory: proline | Vigna aconitifolia |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.75 | - |
2-oxoglutarate | 25°C, pH 8.0 | Vigna aconitifolia | |
| 2 | - |
L-ornithine | 25°C, pH 8.0 | Vigna aconitifolia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48100 | - |
x * 48100, calculated | Vigna aconitifolia |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vigna aconitifolia | P31893 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Vigna aconitifolia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-ornithine + 2-oxoglutarate | - |
Vigna aconitifolia | L-glutamate + DELTA1-pyrroline-5-carboxylate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48100, calculated | Vigna aconitifolia |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Vigna aconitifolia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Vigna aconitifolia |
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Release 2023.1 (January 2023)
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