Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | in rice, cycloheximide, a protein synthesis inhibitor, has no effect on OAT activity induced by water stress | Oryza sativa | |
additional information | OAT activity increases by salt-stress treatment in Arabidopsis thaliana | Arabidopsis thaliana |
Application | Comment | Organism |
---|---|---|
biotechnology | genetic engineering of plants for increased production of the osmoprotectant proline, transgenic plants overexpressing OAT display enhanced tolerance to salt and drought due to increased proline content | Cucurbita pepo |
biotechnology | genetic engineering of plants for increased production of the osmoprotectant proline, transgenic plants overexpressing OAT display enhanced tolerance to salt and drought due to increased proline content | Oryza sativa |
biotechnology | genetic engineering of plants for increased production of the osmoprotectant proline, transgenic plants overexpressing OAT display enhanced tolerance to salt and drought due to increased proline content | Nicotiana plumbaginifolia |
biotechnology | genetic engineering of plants for increased production of the osmoprotectant proline, transgenic plants overexpressing OAT display enhanced tolerance to salt and drought due to increased proline content | Pisum sativum |
biotechnology | genetic engineering of plants for increased production of the osmoprotectant proline, transgenic plants overexpressing OAT display enhanced tolerance to salt and drought due to increased proline content | Arabidopsis thaliana |
biotechnology | genetic engineering of plants for increased production of the osmoprotectant proline, transgenic plants overexpressing OAT display enhanced tolerance to salt and drought due to increased proline content | Vigna aconitifolia |
drug development | human OAT as a potential target for development of new therapeutic drugs, OAT holds a significant scientific interest because of its association with gyrate atrophy, a recessive hereditary genetic dissorder leading to progressive loss of vision and eventually blindness in humans | Homo sapiens |
drug development | human OAT is recognized as a potential target for chemotherapeutic drug development, in a study performed to evaluate the effect of selective blocking of mitosis in human cancer cells, OAT is identified as a protein, which binds the antimitotic drug diazonamide A and it has a role in regulating mitotic cell division | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
a recombinant seedling OAT is obtained by cDNA expression in Escherichia coli and its substrate specificity is measured, the enzyme is found to be strictly specific for L-ornithine showing practically no activity with putrescine, 1,3-diamimopropane and 4-aminobutyrate | Pisum sativum |
expression of the entire human gene in Escherichia coli | Homo sapiens |
mothbean enzyme is expressed in Escherichia coli | Vigna aconitifolia |
Nicotiana plumbaginifolia plants overexpressing OAT from Arabidopsis synthesize more proline than the control plants and show a higher biomass and a higher germination rate under osmotic stress conditions | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
human OAT is crystallized as a recombinant protein obtained by expression of the entire gene in Escherichia coli, the packing of the dimers in the crystal yields a hexameric quaternary structure in which 3 dimers are arranged to form about one turn of a right-handed superhelix, OAT is also crystallized in the presence of L-canaline and gabaculine, co-crystallization of OAT with (2S, 5S)-5-fluoromethylornithine | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Y85I | mutation of Tyr85 in human OAT to Ile decreases the rate of the reaction of the enzyme with ornithine 1000fold and increases that with 4-aminobutyrate 16fold, indicating that Tyr85 is a major determinant of specificity toward ornithine | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2S,5S)-5-fluoromethylornithine | it blocks the enzyme by a suicide reaction (mechanism-based inhibition) leading to a covalent adduct with the cofactor | Homo sapiens | |
5-amino-1,3-cyclohexadienyl carboxylic acid | gabaculine | Arabidopsis thaliana | |
5-amino-1,3-cyclohexadienyl carboxylic acid | gabaculine | Cucurbita pepo | |
5-amino-1,3-cyclohexadienyl carboxylic acid | gabaculine | Homo sapiens | |
5-amino-1,3-cyclohexadienyl carboxylic acid | gabaculine | Nicotiana plumbaginifolia | |
5-amino-1,3-cyclohexadienyl carboxylic acid | gabaculine | Oryza sativa | |
5-amino-1,3-cyclohexadienyl carboxylic acid | gabaculine | Pisum sativum | |
5-amino-1,3-cyclohexadienyl carboxylic acid | gabaculine | Vigna aconitifolia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.75 | - |
2-oxoglutarate | - |
Vigna aconitifolia | |
2 | - |
L-ornithine | - |
Vigna aconitifolia | |
4.7 | - |
L-ornithine | - |
Cucurbita pepo | |
6.3 | - |
2-oxoglutarate | - |
Cucurbita pepo |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
mitochondrion | - |
Cucurbita pepo | 5739 | - |
mitochondrion | - |
Oryza sativa | 5739 | - |
mitochondrion | - |
Nicotiana plumbaginifolia | 5739 | - |
mitochondrion | - |
Pisum sativum | 5739 | - |
mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
mitochondrion | - |
Vigna aconitifolia | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
mothbean enzyme, 1 * 50000 | Vigna aconitifolia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine + 2-oxoglutarate | Homo sapiens | - |
DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? | |
L-ornithine + 2-oxoglutarate | Cucurbita pepo | - |
DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? | |
L-ornithine + 2-oxoglutarate | Oryza sativa | - |
DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? | |
L-ornithine + 2-oxoglutarate | Nicotiana plumbaginifolia | - |
DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? | |
L-ornithine + 2-oxoglutarate | Pisum sativum | - |
DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? | |
L-ornithine + 2-oxoglutarate | Arabidopsis thaliana | - |
DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? | |
L-ornithine + 2-oxoglutarate | Vigna aconitifolia | - |
DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9FNK4 | - |
- |
Cucurbita pepo | - |
- |
- |
Homo sapiens | - |
- |
- |
Nicotiana plumbaginifolia | - |
- |
- |
Oryza sativa | - |
- |
- |
Pisum sativum | B1A0U3 | - |
- |
Vigna aconitifolia | P31893 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine + 2-oxoglutarate | - |
Homo sapiens | DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? | |
L-ornithine + 2-oxoglutarate | - |
Cucurbita pepo | DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? | |
L-ornithine + 2-oxoglutarate | - |
Oryza sativa | DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? | |
L-ornithine + 2-oxoglutarate | - |
Nicotiana plumbaginifolia | DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? | |
L-ornithine + 2-oxoglutarate | - |
Pisum sativum | DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? | |
L-ornithine + 2-oxoglutarate | - |
Arabidopsis thaliana | DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? | |
L-ornithine + 2-oxoglutarate | - |
Vigna aconitifolia | DELTA1-pyrroline-5-carboxylate + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | the crystal structure of the human enzyme is determined, the functional unit of the protein consists of a dimer built from 2 identical subunits, each monomer contains 12 alpha-helices and 14 beta-strands and can be structurally divided into 3 domains: a large 249 residue domain (PLP-binding domain), a small C-terminal domain of 95 residues and an N-terminal segment of 42 residues | Homo sapiens |
monomer | mothbean enzyme, 1 * 50000 | Vigna aconitifolia |
Synonyms | Comment | Organism |
---|---|---|
L-ornithine:2-oxoacid aminotransferase | - |
Homo sapiens |
L-ornithine:2-oxoacid aminotransferase | - |
Cucurbita pepo |
L-ornithine:2-oxoacid aminotransferase | - |
Oryza sativa |
L-ornithine:2-oxoacid aminotransferase | - |
Nicotiana plumbaginifolia |
L-ornithine:2-oxoacid aminotransferase | - |
Pisum sativum |
L-ornithine:2-oxoacid aminotransferase | - |
Arabidopsis thaliana |
L-ornithine:2-oxoacid aminotransferase | - |
Vigna aconitifolia |
OAT | - |
Homo sapiens |
OAT | - |
Cucurbita pepo |
OAT | - |
Oryza sativa |
OAT | - |
Nicotiana plumbaginifolia |
OAT | - |
Pisum sativum |
OAT | - |
Arabidopsis thaliana |
OAT | - |
Vigna aconitifolia |
ornithine delta-aminotransferase | - |
Homo sapiens |
ornithine delta-aminotransferase | - |
Cucurbita pepo |
ornithine delta-aminotransferase | - |
Oryza sativa |
ornithine delta-aminotransferase | - |
Nicotiana plumbaginifolia |
ornithine delta-aminotransferase | - |
Pisum sativum |
ornithine delta-aminotransferase | - |
Arabidopsis thaliana |
ornithine delta-aminotransferase | - |
Vigna aconitifolia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Cucurbita pepo |
8 | - |
- |
Vigna aconitifolia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Homo sapiens | |
pyridoxal 5'-phosphate | - |
Cucurbita pepo | |
pyridoxal 5'-phosphate | - |
Oryza sativa | |
pyridoxal 5'-phosphate | - |
Nicotiana plumbaginifolia | |
pyridoxal 5'-phosphate | - |
Pisum sativum | |
pyridoxal 5'-phosphate | - |
Arabidopsis thaliana | |
pyridoxal 5'-phosphate | - |
Vigna aconitifolia |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme is implicated in salt tolerance in higher plants, enzyme is implicated in proline biosynthesis and accumulation via pyrroline-5-carboxylate | Cucurbita pepo |
physiological function | enzyme is implicated in salt tolerance in higher plants, enzyme is implicated in proline biosynthesis and accumulation via pyrroline-5-carboxylate | Oryza sativa |
physiological function | enzyme is implicated in salt tolerance in higher plants, enzyme is implicated in proline biosynthesis and accumulation via pyrroline-5-carboxylate | Pisum sativum |
physiological function | enzyme is implicated in salt tolerance in higher plants, enzyme is implicated in proline biosynthesis and accumulation via pyrroline-5-carboxylate | Vigna aconitifolia |
physiological function | enzyme is implicated in salt tolerance in higher plants, enzyme is implicated in proline biosynthesis and accumulation via pyrroline-5-carboxylate, OAT is essential for nitrogen recycling from arginine but not for the stress-induced proline accumulation, OAT probably links the degradation pathways for arginine and proline | Arabidopsis thaliana |