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Literature summary for 2.6.1.13 extracted from

  • Jortzik, E.; Fritz-Wolf, K.; Sturm, N.; Hipp, M.; Rahlfs, S.; Becker, K.
    Redox regulation of Plasmodium falciparum ornithine delta-aminotransferase (2010), J. Mol. Biol., 402, 445-459.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
the three-dimensional crystal structure of Plasmodium falciparum OAT at 2.3 A resolution is reported. The overall structure is very similar to that of the human OAT. In plasmodial OAT, the loop involved in substrate binding contains two cysteine residues, which are lacking in human OAT Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
C154S mutant shows drastically reduced activity. Activity can be activated by Trx but not to the same extent as wild-type Plasmodium falciparum
C154S/C163S double mutant shows the same phenotype as mutant C154S Plasmodium falciparum
C163S mutant degrades ornithine with the same specific activity as the wild-type, and the Trx-mediated activation occurres at higher Trx concentrations when compared to OAT wild-type Plasmodium falciparum
C316S transamination of ornithine and 2-oxoglutarate with an activity is reduced to 25-35% of the wild-type. Mutants can be activated by Trx and reaches a specific activity comparable to PfOAT wild-type with Trx Plasmodium falciparum
C350S transamination of ornithine and 2-oxoglutarate with an activity is reduced to 25-35% of the wild-type. Mutants can be activated by Trx and reaches a specific activity comparable to PfOAT wild-type with Trx Plasmodium falciparum
C390S transamination of ornithine and 2-oxoglutarate with an activity is reduced to 25-35% of the wild-type. Mutants can be activated by Trx and reaches a specific activity comparable to PfOAT wild-type with Trx Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
2-oxoglutarate wild-type with PfTrx, pH 7.4, 37°C Plasmodium falciparum
0.7
-
2-oxoglutarate wild-type with PfGrx, pH 7.4, 37°C Plasmodium falciparum
0.9
-
2-oxoglutarate wild-type, pH 7.4, 37°C Plasmodium falciparum
0.9
-
L-ornithine wild-type with PfTrx, pH 7.4, 37°C Plasmodium falciparum
1.2
-
2-oxoglutarate wild-type (OAT in parasite cell extract), pH 7.4, 37°C Plasmodium falciparum
1.5
-
L-ornithine wild-type with PfGrx, pH 7.4, 37°C Plasmodium falciparum
1.6
-
L-ornithine wild-type, pH 7.4, 37°C Plasmodium falciparum
2.3
-
L-ornithine wild-type (OAT in parasite cell extract), pH 7.4, 37°C Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum Q6LFH8
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.3
-
wild-type (OAT in parasite cell extract), pH 7.4, 37°C Plasmodium falciparum
1.9
-
wild-type with PfGrx, pH 7.4, 37°C Plasmodium falciparum
2
-
wild-type, pH 7.4, 37°C Plasmodium falciparum
20.9
-
wild-type with PfTrx, pH 7.4, 37°C Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ornithine + 2-oxoglutarate
-
Plasmodium falciparum DELTA1-pyrroline-5-carboxylate + glutamate
-
?

Synonyms

Synonyms Comment Organism
ornithine delta-aminotransferase
-
Plasmodium falciparum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.5
-
L-ornithine wild-type with PfTrx, pH 7.4, 37°C Plasmodium falciparum
6.1
-
L-ornithine wild-type, pH 7.4, 37°C Plasmodium falciparum
7.7
-
L-ornithine wild-type with PfGrx, pH 7.4, 37°C Plasmodium falciparum