Crystallization (Comment) | Organism |
---|---|
the three-dimensional crystal structure of Plasmodium falciparum OAT at 2.3 A resolution is reported. The overall structure is very similar to that of the human OAT. In plasmodial OAT, the loop involved in substrate binding contains two cysteine residues, which are lacking in human OAT | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
C154S | mutant shows drastically reduced activity. Activity can be activated by Trx but not to the same extent as wild-type | Plasmodium falciparum |
C154S/C163S | double mutant shows the same phenotype as mutant C154S | Plasmodium falciparum |
C163S | mutant degrades ornithine with the same specific activity as the wild-type, and the Trx-mediated activation occurres at higher Trx concentrations when compared to OAT wild-type | Plasmodium falciparum |
C316S | transamination of ornithine and 2-oxoglutarate with an activity is reduced to 25-35% of the wild-type. Mutants can be activated by Trx and reaches a specific activity comparable to PfOAT wild-type with Trx | Plasmodium falciparum |
C350S | transamination of ornithine and 2-oxoglutarate with an activity is reduced to 25-35% of the wild-type. Mutants can be activated by Trx and reaches a specific activity comparable to PfOAT wild-type with Trx | Plasmodium falciparum |
C390S | transamination of ornithine and 2-oxoglutarate with an activity is reduced to 25-35% of the wild-type. Mutants can be activated by Trx and reaches a specific activity comparable to PfOAT wild-type with Trx | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
2-oxoglutarate | wild-type with PfTrx, pH 7.4, 37°C | Plasmodium falciparum | |
0.7 | - |
2-oxoglutarate | wild-type with PfGrx, pH 7.4, 37°C | Plasmodium falciparum | |
0.9 | - |
2-oxoglutarate | wild-type, pH 7.4, 37°C | Plasmodium falciparum | |
0.9 | - |
L-ornithine | wild-type with PfTrx, pH 7.4, 37°C | Plasmodium falciparum | |
1.2 | - |
2-oxoglutarate | wild-type (OAT in parasite cell extract), pH 7.4, 37°C | Plasmodium falciparum | |
1.5 | - |
L-ornithine | wild-type with PfGrx, pH 7.4, 37°C | Plasmodium falciparum | |
1.6 | - |
L-ornithine | wild-type, pH 7.4, 37°C | Plasmodium falciparum | |
2.3 | - |
L-ornithine | wild-type (OAT in parasite cell extract), pH 7.4, 37°C | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q6LFH8 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.3 | - |
wild-type (OAT in parasite cell extract), pH 7.4, 37°C | Plasmodium falciparum |
1.9 | - |
wild-type with PfGrx, pH 7.4, 37°C | Plasmodium falciparum |
2 | - |
wild-type, pH 7.4, 37°C | Plasmodium falciparum |
20.9 | - |
wild-type with PfTrx, pH 7.4, 37°C | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine + 2-oxoglutarate | - |
Plasmodium falciparum | DELTA1-pyrroline-5-carboxylate + glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ornithine delta-aminotransferase | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Plasmodium falciparum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.5 | - |
L-ornithine | wild-type with PfTrx, pH 7.4, 37°C | Plasmodium falciparum | |
6.1 | - |
L-ornithine | wild-type, pH 7.4, 37°C | Plasmodium falciparum | |
7.7 | - |
L-ornithine | wild-type with PfGrx, pH 7.4, 37°C | Plasmodium falciparum |