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Literature summary for 2.6.1.16 extracted from

  • Deng, M.D.; Grund, A.D.; Wassink, S.L.; Peng, S.S.; Nielsen, K.L.; Huckins, B.D.; Burlingame, R.P.
    Directed evolution and characterization of Escherichia coli glucosamine synthase (2006), Biochimie, 88, 419-429.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
A38T mutant shows increased resistance against glucosamine-6-phosphate Escherichia coli
G471S mutant shows increased resistance against glucosamine-6-phosphate Escherichia coli
I271T mutant shows increased resistance against glucosamine-6-phosphate Escherichia coli
I3T mutant shows increased resistance against glucosamine-6-phosphate Escherichia coli
L468P mutant shows increased resistance against glucosamine-6-phosphate Escherichia coli
S449P mutant shows increased resistance against glucosamine-6-phosphate Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
D-glucosamine-6-phosphate 1 mM, about 50% loss of activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
L-glutamine wild type enzyme Escherichia coli
0.2
-
D-fructose 6-phosphate wild type enzyme Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.001
-
mutant enzyme L468P Escherichia coli
0.38
-
mutant enzyme I271T Escherichia coli
0.38
-
mutant enzyme I3T Escherichia coli
0.38
-
mutant enzyme S449P Escherichia coli
0.39
-
wild type enzyme Escherichia coli
0.42
-
mutant enzyme A38T Escherichia coli
0.42
-
mutant enzyme G471S Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + D-fructose 6-phosphate
-
Escherichia coli L-glutamate + D-glucosamine 6-phosphate
-
?

Synonyms

Synonyms Comment Organism
Gfa1
-
Escherichia coli
GlmS
-
Escherichia coli
glucosamine synthase
-
Escherichia coli
glutamine: fructose-6-phosphate amidotransferase
-
Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
activity of the wild type enzyme is not affected by incubation at 50°C for 60 min Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.56
-
glucosamine-6-phosphate wild type enzyme Escherichia coli