Protein Variants | Comment | Organism |
---|---|---|
A38T | mutant shows increased resistance against glucosamine-6-phosphate | Escherichia coli |
G471S | mutant shows increased resistance against glucosamine-6-phosphate | Escherichia coli |
I271T | mutant shows increased resistance against glucosamine-6-phosphate | Escherichia coli |
I3T | mutant shows increased resistance against glucosamine-6-phosphate | Escherichia coli |
L468P | mutant shows increased resistance against glucosamine-6-phosphate | Escherichia coli |
S449P | mutant shows increased resistance against glucosamine-6-phosphate | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucosamine-6-phosphate | 1 mM, about 50% loss of activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
L-glutamine | wild type enzyme | Escherichia coli | |
0.2 | - |
D-fructose 6-phosphate | wild type enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.001 | - |
mutant enzyme L468P | Escherichia coli |
0.38 | - |
mutant enzyme I271T | Escherichia coli |
0.38 | - |
mutant enzyme I3T | Escherichia coli |
0.38 | - |
mutant enzyme S449P | Escherichia coli |
0.39 | - |
wild type enzyme | Escherichia coli |
0.42 | - |
mutant enzyme A38T | Escherichia coli |
0.42 | - |
mutant enzyme G471S | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + D-fructose 6-phosphate | - |
Escherichia coli | L-glutamate + D-glucosamine 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Gfa1 | - |
Escherichia coli |
GlmS | - |
Escherichia coli |
glucosamine synthase | - |
Escherichia coli |
glutamine: fructose-6-phosphate amidotransferase | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
activity of the wild type enzyme is not affected by incubation at 50°C for 60 min | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.56 | - |
glucosamine-6-phosphate | wild type enzyme | Escherichia coli |