Application | Comment | Organism |
---|---|---|
analysis | development of an assay for glucosamine 6-phosphate synthase that measures the production of glucosamine 6-phosphate by either following the consumption of acetyl-CoA spectrophotometrically at 230 nm or quantifying the free thiol with 5,5'-dithio-bis(2-nitrobenzoic acid), i.e. Ellmans reagent in a discontinuous manner. Simple assay method, which can be adapted to 96-well microtiter plate format | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-amino-2-deoxy-D-glucitol 6-phosphate | - |
Escherichia coli | |
2-amino-2-deoxy-D-mannitol 6-phosphate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
L-glutamine | pH 7.5, 37°C | Escherichia coli | |
0.25 | - |
D-fructose 6-phosphate | pH 7.5, 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
development of an assay for glucosamine 6-phosphate synthase that measures the production of glucosamine 6-phosphate by either following the consumption of acetyl-CoA spectrophotometrically at 230 nm or quantifying the free thiol with 5,5'-dithio-bis(2-nitrobenzoic acid), i.e. Ellmans reagent in a discontinuous manner. Simple assay method, which can be adapted to 96-well microtiter plate format | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + D-fructose 6-phosphate | - |
Escherichia coli | L-glutamate + D-glucosamine 6-phosphate | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00067 | - |
2-amino-2-deoxy-D-mannitol 6-phosphate | pH 7.5, 37°C | Escherichia coli | |
0.018 | - |
2-amino-2-deoxy-D-glucitol 6-phosphate | pH 7.5, 37°C | Escherichia coli |