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Literature summary for 2.6.1.16 extracted from

  • Li, Y.; Lopez, P.; Durand, P.; Ouazzani, J.; Badet, B.; Badet-Denisot, M.A.
    An enzyme-coupled assay for amidotransferase activity of glucosamine-6-phosphate synthase (2007), Anal. Biochem., 370, 142-146.
    View publication on PubMed

Application

Application Comment Organism
analysis development of an assay for glucosamine 6-phosphate synthase that measures the production of glucosamine 6-phosphate by either following the consumption of acetyl-CoA spectrophotometrically at 230 nm or quantifying the free thiol with 5,5'-dithio-bis(2-nitrobenzoic acid), i.e. Ellman’s reagent in a discontinuous manner. Simple assay method, which can be adapted to 96-well microtiter plate format Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
2-amino-2-deoxy-D-glucitol 6-phosphate
-
Escherichia coli
2-amino-2-deoxy-D-mannitol 6-phosphate
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
L-glutamine pH 7.5, 37°C Escherichia coli
0.25
-
D-fructose 6-phosphate pH 7.5, 37°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
development of an assay for glucosamine 6-phosphate synthase that measures the production of glucosamine 6-phosphate by either following the consumption of acetyl-CoA spectrophotometrically at 230 nm or quantifying the free thiol with 5,5'-dithio-bis(2-nitrobenzoic acid), i.e. Ellman’s reagent in a discontinuous manner. Simple assay method, which can be adapted to 96-well microtiter plate format Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + D-fructose 6-phosphate
-
Escherichia coli L-glutamate + D-glucosamine 6-phosphate
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00067
-
2-amino-2-deoxy-D-mannitol 6-phosphate pH 7.5, 37°C Escherichia coli
0.018
-
2-amino-2-deoxy-D-glucitol 6-phosphate pH 7.5, 37°C Escherichia coli