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Literature summary for 2.6.1.16 extracted from

  • Luo, C.; Shao, W.; Li, X.; Chen, Z.; Liu, Y.
    Molecular cloning, sequencing, and expression of a L-glutamine D-fructose 6-phosphate amidotransferase gene from Volvariella volvacea (2009), Protein J., 28, 34-43.
    View publication on PubMed
Search for more literature for 2.6.1.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Volvariella volvacea

Inhibitors

Inhibitors Comment Organism Structure
2-amino-2-deoxy-D-glucitol-6-phosphate
-
 Volvariella volvacea
N3-(4-Methoxyfumaroyl)-L-2,3-diaminopropanoic acid
-
 Volvariella volvacea
uridine 5'-diphospho-N-acetylglucosamine
-
 Volvariella volvacea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.55
-
 D-fructose 6-phosphate recombinant enzyme, in 10 mM KCl, 1 mg/ml bovine serum albumin, 20 mM imidazole buffer (pH 6.8), 1 mM EDTA, 1 mM dithiothreitol, and 10% (v/v) glycerol Volvariella volvacea
0.75
-
 L-glutamine recombinant enzyme, in 10 mM KCl, 1 mg/ml bovine serum albumin, 20 mM imidazole buffer (pH 6.8), 1 mM EDTA, 1 mM dithiothreitol, and 10% (v/v) glycerol Volvariella volvacea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77000
-
 4 * 77000, SDS-PAGE Volvariella volvacea
306000
-
 estimated from amino acid sequence Volvariella volvacea
310000
-
 gel filtration Volvariella volvacea

Organism

Organism UniProt Comment Textmining
Volvariella volvacea Q6DLZ8
-
-
Volvariella volvacea V1-1 Q6DLZ8
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification by immobilized Ni2+ affinity column chromatography Volvariella volvacea

Storage Stability

Storage Stability Organism
4°C, 50 mM MOPS (pH 6.5), 1 mM dithiothreitol, 1 mM EDTA, and 10 mM KCl, overnight storage, recombinant enzyme activity is low and no longer evident with preparations losing 60% of their activity Volvariella volvacea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + D-fructose 6-phosphate
-
 Volvariella volvacea L-glutamate + D-glucosamine 6-phosphate
-
 ?
L-glutamine + D-fructose 6-phosphate
-
 Volvariella volvacea V1-1 L-glutamate + D-glucosamine 6-phosphate
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 77000, SDS-PAGE Volvariella volvacea

Synonyms

Synonyms Comment Organism
GFAT
-
 Volvariella volvacea
GlcN-6-P synthase
-
 Volvariella volvacea
glucosamine-6-phosphate synthase
-
 Volvariella volvacea
L-glutamine D-fructose 6-phosphate amidotransferase
-
 Volvariella volvacea
L-glutamine:D-fructose-6-phosphate amidotransferase (hexose-isomerizing)
-
 Volvariella volvacea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Volvariella volvacea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 half-life of 1 h at 45°C Volvariella volvacea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
-
 Volvariella volvacea

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5
-
 Volvariella volvacea

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.085
-
 recombinant enzyme, in 10 mM KCl, 1 mg/ml bovine serum albumin, 20 mM imidazole buffer (pH 6.8), 1 mM EDTA, 1 mM dithiothreitol, and 10% (v/v) glycerol Volvariella volvacea 2-amino-2-deoxy-D-glucitol-6-phosphate
0.12
-
 recombinant enzyme, in 10 mM KCl, 1 mg/ml bovine serum albumin, 20 mM imidazole buffer (pH 6.8), 1 mM EDTA, 1 mM dithiothreitol, and 10% (v/v) glycerol Volvariella volvacea N3-(4-Methoxyfumaroyl)-L-2,3-diaminopropanoic acid
0.16
-
 recombinant enzyme, in 10 mM KCl, 1 mg/ml bovine serum albumin, 20 mM imidazole buffer (pH 6.8), 1 mM EDTA, 1 mM dithiothreitol, and 10% (v/v) glycerol Volvariella volvacea uridine 5'-diphospho-N-acetylglucosamine