Crystallization (Comment) | Organism |
---|---|
mutant I50Q, V241A, E211S | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E211S | crystallization data, decrease in kcat, decrease in Km-value for 2-oxoglutarate | Escherichia coli |
E211S/I50G | drastic decrease in kcat-value | Escherichia coli |
E211S/I50G/C77K | 10fold increase in decarboxylation activity | Escherichia coli |
E211S/I50G/C77R | drastic decrease in kcat-value | Escherichia coli |
E211S/I50H/V80D | 10fold increase in decarboxylation activity, change of reaction specificity to that of a decarboxylase | Escherichia coli |
E211S/I50H/V80T | decrease in kcat, decrease in Km-value for 2-oxoglutarate | Escherichia coli |
E211S/I50N/V80D | drastic decrease in kcat-value | Escherichia coli |
E211S/I50N/V80T | drastic decrease in kcat-value | Escherichia coli |
E211S/I50Q/G295Y/V241A | drastic decrease in kcat-value | Escherichia coli |
I50Q | crystallization data, decrease in kcat, increase in Km-values | Escherichia coli |
I50Q/G295Y | decrease in kcat, decrease in Km-value for 2-oxoglutarate | Escherichia coli |
V241A | crystallization data, decrease in kcat, decrease in Km-value for 2-oxoglutarate | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | substrate inhibition | Escherichia coli | |
Aminooxyacetate | - |
Escherichia coli | |
Glutarate | - |
Escherichia coli | |
Maleate | - |
Escherichia coli | |
additional information | no substrate inhibition: 4-aminobutanoate | Escherichia coli | |
succinate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0021 | - |
2-oxoglutarate | mutant E211S, 25°C, pH 7.8 | Escherichia coli | |
0.063 | - |
2-oxoglutarate | mutant E211S/I50H/V80T, 25°C, pH 7.8 | Escherichia coli | |
0.13 | - |
2-oxoglutarate | mutant V241A, 25°C, pH 7.8 | Escherichia coli | |
0.72 | - |
2-oxoglutarate | mutant E211S/I50G/C77K, 25°C, pH 7.8 | Escherichia coli | |
0.78 | - |
2-oxoglutarate | mutant I50Q/G295Y, 25°C, pH 7.8 | Escherichia coli | |
1.07 | - |
2-oxoglutarate | wild-type, 25°C, pH 7.8 | Escherichia coli | |
4.7 | - |
4-aminobutanoate | mutant I50Q/G295Y, 25°C, pH 7.8 | Escherichia coli | |
5.8 | - |
4-aminobutanoate | wild-type, 25°C, pH 7.8 | Escherichia coli | |
5.9 | - |
4-aminobutanoate | mutant E211S/I50G/C77K, 25°C, pH 7.8 | Escherichia coli | |
30 | - |
4-aminobutanoate | mutant V241A, 25°C, pH 7.8 | Escherichia coli | |
46 | - |
2-oxoglutarate | mutant I50Q, 25°C, pH 7.8 | Escherichia coli | |
68 | - |
4-aminobutanoate | mutant I50Q, 25°C, pH 7.8 | Escherichia coli | |
86 | - |
4-aminobutanoate | mutant E211S, 25°C, pH 7.8 | Escherichia coli | |
146 | - |
4-aminobutanoate | mutant E211S/I50H/V80T, 25°C, pH 7.8 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P22256 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobutanoate + 2-oxoglutarate | - |
Escherichia coli | 4-oxobutanoate + L-glutamate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00023 | - |
4-aminobutanoate | mutant E211S/I50H/V80D, 25°C, pH 7.8 | Escherichia coli | |
0.0005 | - |
4-aminobutanoate | mutant E211S/I50G/C77R, 25°C, pH 7.8 | Escherichia coli | |
0.003 | - |
4-aminobutanoate | mutant E211S/I50G, 25°C, pH 7.8 | Escherichia coli | |
0.004 | - |
4-aminobutanoate | mutant E211S/I50Q/G295Y/V241A, 25°C, pH 7.8 | Escherichia coli | |
0.0058 | - |
4-aminobutanoate | mutant E211S/I50N/V80T, 25°C, pH 7.8 | Escherichia coli | |
0.0075 | - |
4-aminobutanoate | mutant E211S/I50N/V80D, 25°C, pH 7.8 | Escherichia coli | |
0.028 | - |
4-aminobutanoate | mutant I50Q/G295Y, 25°C, pH 7.8 | Escherichia coli | |
0.106 | - |
4-aminobutanoate | mutant E211S/I50H/V80T, 25°C, pH 7.8 | Escherichia coli | |
0.25 | - |
4-aminobutanoate | mutant E211S/I50G/C77K, 25°C, pH 7.8 | Escherichia coli | |
0.56 | - |
4-aminobutanoate | mutant E211S, 25°C, pH 7.8 | Escherichia coli | |
1.93 | - |
4-aminobutanoate | mutant V241A, 25°C, pH 7.8 | Escherichia coli | |
13.5 | - |
4-aminobutanoate | mutant I50Q, 25°C, pH 7.8 | Escherichia coli | |
47.4 | - |
4-aminobutanoate | wild-type, 25°C, pH 7.8 | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
Aminooxyacetate | - |
Escherichia coli | |
0.62 | - |
Aminooxyacetate | wild-type, pH 7.8, 25°C | Escherichia coli | |
1.13 | - |
Aminooxyacetate | mutant V241A, pH 7.8, 25°C | Escherichia coli | |
3.9 | - |
Maleate | wild-type, pH 7.8, 25°C | Escherichia coli | |
19 | - |
Glutarate | wild-type, pH 7.8, 25°C | Escherichia coli | |
27 | - |
succinate | wild-type, pH 7.8, 25°C | Escherichia coli | |
46 | - |
Maleate | mutant I50Q, pH 7.8, 25°C | Escherichia coli | |
140 | - |
Glutarate | mutant V241A, pH 7.8, 25°C | Escherichia coli | |
197 | - |
succinate | mutant I50Q, pH 7.8, 25°C | Escherichia coli | |
233 | - |
Glutarate | mutant I50Q, pH 7.8, 25°C | Escherichia coli | |
720 | - |
succinate | mutant V241A, pH 7.8, 25°C | Escherichia coli | |
780 | - |
Maleate | mutant V241A, pH 7.8, 25°C | Escherichia coli |