Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Aminooxyacetate | 1 mM, 98% inhibition | Hordeum vulgare | |
Glutarate | 10 mM, 94% inhibition | Hordeum vulgare | |
Semicarbazide | 10 mM, 97% inhibition | Hordeum vulgare |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
pyruvate | pH 8.0, 23°C | Hordeum vulgare | |
1 | - |
glutamate | pH 8.0, 23°C | Hordeum vulgare | |
5 | - |
2-oxoglutarate | pH 8.0, 23°C | Hordeum vulgare | |
17 | - |
alanine | pH 8.0, 23°C | Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Hordeum vulgare |
97000 | - |
gel filtration | Hordeum vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
4 isoforms | - |
Purification (Comment) | Organism |
---|---|
anaerobically induced isoform, ammonium sulfate, gel filtration, Q-Sepharose, Mono P, Mono Q | Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Hordeum vulgare | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2231 | - |
- |
Hordeum vulgare |
Storage Stability | Organism |
---|---|
-20°C, 10% glycerol, half-life of 2 weeks | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + 2-oxoglutarate | highly specific for alanine and 2-oxoglutarate | Hordeum vulgare | pyruvate + L-glutamate | - |
? | |
L-alanine + 2-oxoglutarate | cannot use glyoxylate as keto donor | Hordeum vulgare | pyruvate + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.3 | with alanine and 2-oxoglutarate | Hordeum vulgare |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | anaerobically induced isoform does not require the presence of pyridoxal 5'-phosphate to retain its activity | Hordeum vulgare |