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Literature summary for 2.6.1.2 extracted from

  • Srivastava, A.S.; Oohara, I.; Suzuki, T.; Shenouda, S.; Singh, S.N.; Chauhan, D.P.; Carrier, E.
    Purification and properties of cytosolic alanine aminotransferase from the liver of two freshwater fish, Clarias batrachus and Labeo rohita (2004), Comp. Biochem. Physiol. B, 137, 197-207.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Aminooxyacetate competitive to L-alanine, uncompetitive to 2-oxoglutarate Clarias batrachus
Aminooxyacetate competitive to L-alanine, uncompetitive to 2-oxoglutarate Labeo rohita

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.29
-
2-oxoglutarate pH 7.4, 25°C Clarias batrachus
0.33
-
2-oxoglutarate pH 7.4, 25°C Labeo rohita
18.5
-
L-alanine pH 7.4, 25°C Clarias batrachus
23.55
-
L-alanine pH 7.4, 25°C Labeo rohita

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Clarias batrachus 5829
-
cytosol
-
Labeo rohita 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
x * 54000 + x * 56000, SDS-PAGE Clarias batrachus
54000
-
x * 54000 + x * 56000, SDS-PAGE Labeo rohita
56000
-
x * 54000 + x * 56000, SDS-PAGE Clarias batrachus
56000
-
x * 54000 + x * 56000, SDS-PAGE Labeo rohita

Organism

Organism UniProt Comment Textmining
Clarias batrachus
-
-
-
Labeo rohita
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Clarias batrachus
-
liver
-
Labeo rohita
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
50
-
pH 7.4, 25°C Labeo rohita
89.5
-
pH 7.4, 25°C Clarias batrachus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + 2-oxoglutarate
-
Clarias batrachus pyruvate + L-glutamate
-
?
L-alanine + 2-oxoglutarate
-
Labeo rohita pyruvate + L-glutamate
-
?

Subunits

Subunits Comment Organism
? x * 54000 + x * 56000, SDS-PAGE Clarias batrachus
? x * 54000 + x * 56000, SDS-PAGE Labeo rohita

Synonyms

Synonyms Comment Organism
c-AAT
-
Clarias batrachus
c-AAT
-
Labeo rohita

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Clarias batrachus
7.5
-
-
Labeo rohita

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00034
-
Aminooxyacetate pH 7.4, 25°C Labeo rohita
0.00064
-
Aminooxyacetate pH 7.4, 25°C Clarias batrachus