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Literature summary for 2.6.1.2 extracted from
- Isolation and characterization of cytosolic alanine aminotransferase isoforms from starved rat liver (2004), Mol. Cell. Biochem., 267, 13-23.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Aminooxyacetate | complete inhibition, both isoforms | Rattus norvegicus |  |
| Glutarate | about 70% inhibition, both isoforms | Rattus norvegicus | |
| HgCl2 | almost complete inhibition, both isoforms | Rattus norvegicus | |
| hydroxylamine | complete inhibition, both isoforms | Rattus norvegicus | |
| Maleate | about 70% inhibition, both isoforms | Rattus norvegicus | |
| o-phenanthroline | weak | Rattus norvegicus | |
| p-hydroxymercuribenzoate | complete inhibition, both isoforms | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17700 | - |
x * 17700, isoform AlaAT I, x * 112200, isoform AlaAT II, SDS-PAGE | Rattus norvegicus |
| 18370 | - |
isoform AlaAT I, gel filtration | Rattus norvegicus |
| 112200 | - |
x * 17700, isoform AlaAT I, x * 112200, isoform AlaAT II, SDS-PAGE | Rattus norvegicus |
| 114800 | - |
isoform AlaAT II, gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male animals, under starvation, isoforms AlaAT I and AlaAT II | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 30.58 | - |
isoform AlaAT I | Rattus norvegicus |
| 43.3 | - |
isoform AlaAT II | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DL-alanine + 2-oxoglutarate | 63-70% of the activity with L-alanine | Rattus norvegicus | pyruvate + L-glutamate | - |
? | |
| L-alanine + 2-oxoglutarate | - |
Rattus norvegicus | pyruvate + L-glutamate | - |
? | |
| L-aspartate + 2-oxoglutarate | 6-8% of the activity with L-alanine | Rattus norvegicus | 2-oxobutan-1,4-dioate + L-glutamate | - |
? | |
| L-leucine + 2-oxoglutarate | 8% of the activity with L-alanine | Rattus norvegicus | 2-oxo-4-methylpentanoate + L-glutamate | - |
? | |
| L-tyrosine + 2-oxoglutarate | 12-14% of the activity with L-alanine | Rattus norvegicus | 2-deamino-2-oxotyrosine + L-glutamate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | x * 17700, isoform AlaAT I, x * 112200, isoform AlaAT II, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AlaAT | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Rattus norvegicus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Rattus norvegicus | isoform AlaAT I, isoelectric focusing | - |
4.2 |
| Rattus norvegicus | isoform AlaAT II, isoelectric focusing | - |
5.3 |
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Release 2023.1 (January 2023)
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