Literature summary for 2.6.1.21 extracted from

Kishimoto, K.; Yoshimura, T.; Esaki, N.; Sugio, S.; Manning, J.M.; Soda, K.
Role of leucine 201 of thermostable D-amino acid aminotransferase from a thermophile, Bacillus sp. YM-1 (1995), J. Biochem., 117, 691-696.

Search for more literature for 2.6.1.21

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type, L201A and L201W mutant enzymes in Escherichia coli	Bacillus sp. (in: Bacteria)

Protein Variants

Protein Variants	Comment	Organism
L201A	2% of wild-type kcat	Bacillus sp. (in: Bacteria)
L201W	0.043% of wild-type kcat	Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	L201A and L201W mutant enzymes lose their activity by incubation with D-alanine with biphasic kinetics	Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.63	-	2-oxoglutarate	pH 8.0, 37°C, wild-type enzyme	Bacillus sp. (in: Bacteria)
0.67	-	2-oxoglutarate	pH 8.0, 37°C, L201W mutant enzyme	Bacillus sp. (in: Bacteria)
1.2	-	D-alanine	pH 8.0, 37°C, L201W mutant enzyme	Bacillus sp. (in: Bacteria)
1.5	-	D-alanine	pH 8.0, 37°C, L201A mutant enzyme	Bacillus sp. (in: Bacteria)
4.8	-	2-oxoglutarate	pH 8.0, 37°C, L201A mutant enzyme	Bacillus sp. (in: Bacteria)
20	-	D-alanine	pH 8.0, 37°C, wild-type enzyme	Bacillus sp. (in: Bacteria)

Organism

Organism	UniProt	Comment	Textmining
Bacillus sp. (in: Bacteria)	-	YM-1, thermophile	-
Bacillus sp. (in: Bacteria) YM-1	-	YM-1, thermophile	-

Purification (Commentary)

Purification (Comment)	Organism
wild-type, L201A and L201W mutant enzyme, DEAE-toyopearl	Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-alanine + 2-oxoglutarate	-	Bacillus sp. (in: Bacteria)	pyruvate + D-glutamate	-	r
D-alanine + 2-oxoglutarate	-	Bacillus sp. (in: Bacteria) YM-1	pyruvate + D-glutamate	-	r

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.12	-	D-alanine	pH 8.0, 37°C, L201W mutant enzyme	Bacillus sp. (in: Bacteria)
5.7	-	D-alanine	pH 8.0, 37°C, L201A mutant enzyme	Bacillus sp. (in: Bacteria)
280	-	D-alanine	pH 8.0, 37°C, wild-type enzyme	Bacillus sp. (in: Bacteria)

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	wild-type, L201A and L201W mutant enzyme: 0.89, 0.78 and 0.56 mol per mol of subunit, respectively	Bacillus sp. (in: Bacteria)

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)