Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | in excess, substrate inhibition | Sus scrofa | |
pyridoxal 5'-phosphate | inhibits at high concentrations, activates at low concentrations | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.185 | - |
pH 7.5, 37°C | Sus scrofa |
Storage Stability | Organism |
---|---|
-20°C, in 0.05 M phosphate buffer, pH 7.5, at least 3 weeks | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-amino-2-methylpropanoate + 2-oxoglutarate | i.e. L-beta-aminoisobutyrate, not D-isomer | Sus scrofa | 2-methyl-3-oxopropanoate + L-glutamate | - |
r | |
4-aminobutanoate + 2-oxoglutarate | transamination at 92% the rate of (S)-3-amino-2-methylpropanoate | Sus scrofa | succinic semialdehyde + L-glutamate | - |
? | |
5-aminopentanoate + 2-oxoglutarate | transamination at 33% the rate of (S)-3-amino-2-methylpropanoate | Sus scrofa | 5-oxopentanoate + L-glutamate | - |
? | |
beta-alanine + 2-oxoglutarate | as good as (S)-3-amino-2-methylpropanoate | Sus scrofa | malonic semialdehyde + L-glutamate | - |
? | |
additional information | no substrates are 3-aminobutanoate, 2-ethyl-beta-alanine, glycine, L-alpha-alanine, 2-aminoisobutanoate, lysine, taurine, O-phosphoethanolamine, homocarnosine. Poor substrates are 6-aminohexanoate or ornithine, no substrates are pyruvate, oxaloacetate or glyoxylate | Sus scrofa | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.1 | - |
- |
Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9.5 | about half-maximal activity at pH 8.5 and 9.5 | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | activates at low concentrations, inhibits at high concentrations | Sus scrofa |