Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | absolutely required | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-hydroxymercuribenzoate | complete inhibition at 0.1 mM | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
L-triiodothyronine | pH 7.8, 37°C | Oryctolagus cuniculus | |
0.2 | - |
2-oxoglutarate | cosubstrate triiodothyronine, pH 7.8, 37°C | Oryctolagus cuniculus | |
0.2 | - |
2-oxoglutarate | cosubstrate L-thyroxine, pH 7.8, 37°C | Oryctolagus cuniculus | |
0.9 | - |
3,5-diiodo-L-tyrosine | pH 7.8, 37°C | Oryctolagus cuniculus | |
2 | - |
pyruvate | cosubstrate L-triiodothyronine, pH 7.8, 37°C | Oryctolagus cuniculus | |
2 | - |
2-oxoglutarate | cosubstrate 3,5-dinitro-L-tyrosine, pH 7.8, 37°C | Oryctolagus cuniculus | |
2.12 | - |
L-thyronine | pH 7.8, 37°C | Oryctolagus cuniculus | |
3.8 | - |
3-iodo-L-tyrosine | pH 7.8, 37°C | Oryctolagus cuniculus | |
4.4 | - |
3,5-Dinitro-L-tyrosine | pH 7.8, 37°C | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Oryctolagus cuniculus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
2 * 48000, SDS-PAGE | Oryctolagus cuniculus |
95000 | - |
glycerol density gradient centrifugation | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-3,5,3'-triiodothyronine + 2-oxoglutarate | Oryctolagus cuniculus | - |
3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate | i.e. 3,5,3'-triiodothyropyruvate + L-Glu | r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | low activity | Oryctolagus cuniculus | - |
heart | low activity | Oryctolagus cuniculus | - |
kidney | - |
Oryctolagus cuniculus | - |
liver | - |
Oryctolagus cuniculus | - |
lung | low activity | Oryctolagus cuniculus | - |
additional information | not in serum | Oryctolagus cuniculus | - |
skeletal muscle | low activity | Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.013 | - |
purified enzyme, substrates L-3,5,3'-triiodothyronine + 2-oxoglutarate | Oryctolagus cuniculus |
15 | - |
purified enzyme, substrates 3,5-dinitro-L-tyrosine + 2-oxoglutarate | Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
-20°C, 30% loss of activity per month | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,5-dibromo-L-tyrosine + 2-oxoglutarate | - |
Oryctolagus cuniculus | 3-(3,5-dibromo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate | - |
r | |
3,5-diiodo-D-tyrosine + 2-oxoglutarate | very low activity | Oryctolagus cuniculus | 3-(3,5-diiodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate | - |
r | |
3,5-diiodo-L-tyrosine + 2-oxoglutarate | - |
Oryctolagus cuniculus | 3-(3,5-diiodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate | - |
r | |
3,5-dinitro-L-tyrosine + 2-oxoglutarate | - |
Oryctolagus cuniculus | 3-(3,5-dinitro-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate | i.e. 3,5-dinitro-p-hydroxyphenylpyruvic acid + Glu | r | |
3-amino-L-tyrosine + 2-oxoglutarate | very low activity | Oryctolagus cuniculus | 3-(3-amino-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate | - |
r | |
3-iodo-L-tyrosine + 2-oxoglutarate | - |
Oryctolagus cuniculus | 3-(3-iodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate | - |
r | |
3-nitro-L-tyrosine + 2-oxoglutarate | - |
Oryctolagus cuniculus | 3-(3-nitro-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate | - |
r | |
L-3,5,3'-triiodothyronine + 2-oxoglutarate | - |
Oryctolagus cuniculus | 3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate | i.e. 3,5,3'-triiodothyropyruvate + L-Glu | r | |
L-3,5,3'-triiodothyronine + oxaloacetate | - |
Oryctolagus cuniculus | 3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + glycine | i.e. 3,5,3'-triiodothyrophenylpyruvate + Gly | r | |
L-3,5,3'-triiodothyronine + pyruvate | - |
Oryctolagus cuniculus | 3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-alanine | i.e. 3,5,3'-triiodothyrophenylpyruvate + L-Ala | r | |
L-phenylalanine + 2-oxoglutarate | low activtiy | Oryctolagus cuniculus | 3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate | - |
r | |
L-thyronine + 2-oxoglutarate | - |
Oryctolagus cuniculus | ? | - |
? | |
L-thyroxine + 2-oxoglutarate | - |
Oryctolagus cuniculus | 3-[4-(4-hydroxy-3,5-diiodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate | - |
r | |
L-tryptophan + 2-oxoglutarate | low activtiy | Oryctolagus cuniculus | 3-indole-2-oxopropanoate + L-glutamate | - |
r | |
L-tyrosine + 2-oxoglutarate | low activtiy | Oryctolagus cuniculus | 2-oxo-3-phenylpropanoate + L-glutamate | - |
r | |
additional information | specific for 2-oxoglutarate | Oryctolagus cuniculus | ? | - |
? | |
additional information | pyruvate and oxalacetate are ineffective with 3,5-dinitro-L-tyrosine and only partially effective with iodinated tyrosines | Oryctolagus cuniculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 48000, SDS-PAGE | Oryctolagus cuniculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0233 | - |
L-thyroxine | pH 7.8, 37°C | Oryctolagus cuniculus | |
0.0317 | - |
L-triiodothyronine | pH 7.8, 37°C | Oryctolagus cuniculus | |
1.1 | - |
3,5-diiodo-L-tyrosine | pH 7.8, 37°C | Oryctolagus cuniculus | |
3.68 | - |
3-iodo-L-tyrosine | pH 7.8, 37°C | Oryctolagus cuniculus | |
6.6 | - |
3,5-diiodo-L-tyrosine | pH 7.8, 37°C | Oryctolagus cuniculus | |
39.9 | - |
3,5-Dinitro-L-tyrosine | pH 7.8, 37°C | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 8.6 | broad optimum | Oryctolagus cuniculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.6 | pH 7.0: 30% of maximal activity with L-triiodothyronine, 15% of maximal activity with 3,5-dinitro-L-tyrosine, pH 7.4-8.6: maximal activity with triiodothyronine and 3,5-dinitro-L-tyrosine | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | partial requirement | Oryctolagus cuniculus |