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Literature summary for 2.6.1.28 extracted from

  • Koide, Y.; Honma, M.; Shimomura, T.
    L-tryptophan-alpha-ketoisocaproate aminotransferase from Pseudomonas sp. (1980), Agric. Biol. Chem., 44, 2013-2019.
No PubMed abstract available
Search for more literature for 2.6.1.28

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 2-Oxoisohexanoate with L-tryptophan, pH 8.0, 30°C Pseudomonas sp.
0.08
-
 2-Oxohexanoate with tryptophan, pH 8.0, 30°C Pseudomonas sp.
0.11
-
 2-oxoisopentanoate with L-tryptophan, pH 8.0, 30°C Pseudomonas sp.
0.12
-
 2-oxopentanoate with L-tryptophan, pH 8.0, 30°C Pseudomonas sp.
0.2
-
 Indolepyruvate with L-leucine, pH 8.0, 30°C Pseudomonas sp.
0.4
-
 L-leucine with phenylpyruvate, pH 8.0, 30°C Pseudomonas sp.
0.47
-
 L-isoleucine with phenylpyruvate, pH 8.0, 30°C Pseudomonas sp.
0.8
-
 L-phenylalanine with 2-oxoisohexanoate, pH 8.0, 30°C Pseudomonas sp.
1
-
 L-tyrosine with 2-oxoisohexanoate, pH 8.0, 30°C Pseudomonas sp.
1.4
-
 2-oxobutanoate with L-tryptophan, pH 8.0, 30°C Pseudomonas sp.
1.4
-
 L-tryptophan with 2-oxoisohexanoate, pH 8.0, 30°C Pseudomonas sp.
3.4
-
 L-norleucine with phenylpyruvate, pH 8.0, 30°C Pseudomonas sp.
4.5
-
 L-valine with phenylpyruvate, pH 8.0, 30°C Pseudomonas sp.
4.5
-
 L-norvaline with phenylpyruvate, pH 8.0, 30°C Pseudomonas sp.
12
-
 pyruvate with L-tryptophan, pH 8.0, 30°C Pseudomonas sp.
23.8
-
 L-histidine with 2-oxoisohexanoate, pH 8.0, 30°C Pseudomonas sp.
30
-
 L-2-aminobutanoate with phenylpyruvate, pH 8.0, 30°C Pseudomonas sp.
250
-
 L-alanine with 2-oxoisohexanoate, pH 8.0, 30°C Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 sedimentation equilibrium analysis Pseudomonas sp.
110000
-
 gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
76
-
 purified enzyme Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-aminobutanoate + phenylpyruvate
-
 Pseudomonas sp. L-phenylalanine + 2-oxobutanoate
-
 ?
L-histidine + 2-oxoisohexanoate
-
 Pseudomonas sp. ?
-
 ?
L-isoleucine + phenylpyruvate
-
 Pseudomonas sp. L-phenylalanine + 3-methyl-2-oxopentanoate
-
 ?
L-phenylalanine + 2-oxobutanoate low activity Pseudomonas sp. phenylpyruvate + 2-aminobutanoate
-
 r
L-phenylalanine + 2-oxohexanoate
-
 Pseudomonas sp. phenylpyruvate + L-norleucine
-
 r
L-phenylalanine + 2-oxoisohexanoate i.e alpha-ketoisocaproate, highest activity as amino acceptor Pseudomonas sp. phenylpyruvate + L-leucine
-
 r
L-phenylalanine + 2-oxoisopentanoate
-
 Pseudomonas sp. phenylpyruvate + L-valine
-
 r
L-phenylalanine + pyruvate 2% of the activity with alpha-ketoisocaproate Pseudomonas sp. phenylpyruvate + L-alanine
-
 r
L-tryptophan + 2-oxobutanoate weak activity Pseudomonas sp. 3-indole-2-oxopropanoate + 2-aminobutanoate
-
 r
L-tryptophan + 2-oxoglutarate no activity Pseudomonas sp. 3-indole-2-oxopropanoate + L-glutamate
-
 ?
L-tryptophan + 2-oxohexanoate
-
 Pseudomonas sp. 3-indole-2-oxopropanoate + L-norleucine
-
 r
L-tryptophan + 2-oxoisohexanoate
-
 Pseudomonas sp. 3-indole-2-oxopropanoate + L-leucine
-
 r
L-tryptophan + 2-oxoisopentanoate i.e. L-Trp + dimethylpyruvate Pseudomonas sp. 3-indole-2-oxopropanoate + L-valine
-
 r
L-tryptophan + 2-oxopentanoate
-
 Pseudomonas sp. 3-indole-2-oxopropanoate + norvaline
-
 r
L-tryptophan + phenylpyruvate
-
 Pseudomonas sp. 3-indole-2-oxopropanoate + L-phenylalanine i.e. indolepyruvate + L-Phe r
L-tryptophan + pyruvate 2% of the activity with alpha-ketoisocaproate Pseudomonas sp. 3-indole-2-oxopropanoate + L-alanine
-
 r
additional information no activity with glutamate, 2-oxoglutarate Pseudomonas sp. ?
-
 ?
phenylpyruvate + DL-5-hydroxytryptophan
-
 Pseudomonas sp. phenylalanine + 3-(5-hydroxyindole)-2-oxopropanoate
-
 r
phenylpyruvate + DL-isoleucine
-
 Pseudomonas sp. phenylalanine + 3-methyl-2-oxopentanoate
-
 r
phenylpyruvate + DL-leucine
-
 Pseudomonas sp. phenylalanine + 4-methyl-2-oxopentanoate
-
 r
phenylpyruvate + DL-methionine low activity Pseudomonas sp. phenylalanine + 4-methylsulfanyl-2-oxobutanoate
-
 r
phenylpyruvate + DL-valine
-
 Pseudomonas sp. phenylalanine + 3-methyl-2-oxobutanoate
-
 r
phenylpyruvate + L-2-iminobutanoate
-
 Pseudomonas sp. phenylalanine + ?
-
 r
phenylpyruvate + L-citrulline low activity Pseudomonas sp. phenylalanine + 2-oxo-5-ureido-pentanoate
-
 r
phenylpyruvate + L-norleucine
-
 Pseudomonas sp. phenylalanine + 2-oxohexanoate
-
 r
phenylpyruvate + L-tyrosine
-
 Pseudomonas sp. phenylalanine + 3-(4-hydroxyphenyl)-2-oxopropanoate
-
 r
phenylpyruvate + norvaline
-
 Pseudomonas sp. phenylalanine + 2-oxopentanoate
-
 r
phenylpyruvate + phenylglycine
-
 Pseudomonas sp. phenylalanine + phenylglyoxylate
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Pseudomonas sp.