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Literature summary for 2.6.1.36 extracted from

  • Soda, K.; Misono, H.
    L-Lysine-ketoglutarate aminotransferase (Acetobacter liquidum) (1971), Methods Enzymol., 17B, 222-228.
No PubMed abstract available
Search for more literature for 2.6.1.36

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 [Flavobacterium] lutescens

Inhibitors

Inhibitors Comment Organism Structure
5-Aminopentanoate
-
 [Flavobacterium] lutescens
5-hydroxylysine DL- [Flavobacterium] lutescens
O-(2-aminoethyl)-DL serine
-
 [Flavobacterium] lutescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 2-oxoglutarate cosubstrate L-ornithine, pH 8.0, 37°C [Flavobacterium] lutescens
0.5
-
 2-oxoglutarate L-lysine, pH 8.0, 37°C [Flavobacterium] lutescens
2
-
 L-ornithine pH 8.0, 37°C [Flavobacterium] lutescens
2
-
 S-(2-aminoethyl)-L-cysteine pH 8.0, 37°C [Flavobacterium] lutescens
2.8
-
 L-lysine pH 8.0, 37°C [Flavobacterium] lutescens
3.8
-
 2-oxoglutarate pH 8.0, 37°C [Flavobacterium] lutescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
116000
-
 sedimentation equilibrium method [Flavobacterium] lutescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + 2-oxoglutarate [Flavobacterium] lutescens
-
 2-aminoadipate 6-semialdehyde + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
[Flavobacterium] lutescens
-
 i.e. Achromobacter liquidum
-

Purification (Commentary)

Purification (Comment) Organism
-
 [Flavobacterium] lutescens

Storage Stability

Storage Stability Organism
0-5°C, crystalline enzyme, 0.2 M potassium phosphate buffer, pH 7.2, 50% saturation of ammonium sulfate, 0.1 mM pyridoxal 5'-phosphate, 0.01% 2-mercaptoethanol, stable for several weeks [Flavobacterium] lutescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-N-acetyl-L-ornithine + 2-oxoglutarate 4.0% of the activity with L-lysine [Flavobacterium] lutescens 5-(N-acetylamino)-2-oxopentanoate + L-glutamate
-
 ?
6-N-acetyl-L-lysine + 2-oxoglutarate 5.7% of the activity with L-lysine [Flavobacterium] lutescens 6-(N-acetylamino)-2-oxohexanoate + L-glutamate
-
 ?
L-lysine + 2-oxobutanoate 5.0% of the activity with 2-oxoglutarate [Flavobacterium] lutescens 2-aminoadipate 6-semialdehyde + 2-aminobutanoate
-
 ?
L-lysine + 2-oxoglutarate
-
 [Flavobacterium] lutescens 2-aminoadipate 6-semialdehyde + L-glutamate
-
 ?
L-lysine + 2-oxoglutarate
-
 [Flavobacterium] lutescens 2-aminoadipate 6-semialdehyde + L-glutamate the product allysine is converted into the intramolecularly dehydrated form 1-piperideine 6-carboxylate ?
L-lysine + 2-oxohexanoate 8.7% of the activity with 2-oxoglutarate [Flavobacterium] lutescens 2-aminoadipate 6-semialdehyde + 2-aminohexanoate
-
 ?
L-lysine + 2-oxopentanoate 5.3% of the activity with 2-oxoglutarate [Flavobacterium] lutescens 2-aminoadipate 6-semialdehyde + 2-aminopentanoate
-
 ?
L-lysine + oxaloacetate 5.3% of the activity with 2-oxoglutarate [Flavobacterium] lutescens 2-aminoadipate 6-semialdehyde + L-aspartate
-
 ?
L-ornithine + 2-oxoglutarate 55% of the activity with L-lysine [Flavobacterium] lutescens ? + L-glutamate
-
 ?
additional information no substrate: D-lysine, 2-N-acetyl-L-lysine, 2-N-acetyl-L-ornithine, S-(2-aminoethyl)alpha-L-cysteine [Flavobacterium] lutescens ?
-
 ?
S-(2-aminoethyl)-L-cysteine + 2-oxoglutarate
-
 [Flavobacterium] lutescens ? + L-glutamate
-
 ?
S-(2-N-acetylaminoethyl)-L-cysteine + 2-oxoglutarate 3.3% of the activity with L-lysine [Flavobacterium] lutescens S-(2-N-acetylaminoethyl)-2-oxopropanoic acid + L-glutamate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 [Flavobacterium] lutescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 substrates L-ornithine + 2-oxoglutarate [Flavobacterium] lutescens
8.3 8.5 substrates lysine + 2-oxoglutarate [Flavobacterium] lutescens
9
-
 substrate S-(2-aminoethyl)-L-cysteine [Flavobacterium] lutescens