Crystallization (Comment) | Organism |
---|---|
crystal structures of AAA-AT in four forms: with pyridoxal 5'-phosphate (PLP) (PLP complex), with PLP and leucine (PLP/Leu complex), with N-phosphopyridoxyl-leucine (PPL) (PPL complex), and with N-phosphopyridoxyl-alpha-aminoadipate (PPA) at 2.67, 2.26, 1.75, and 1.67 A resolution, respectively | Thermus thermophilus |
in complex with pyridoxal 5'-phosphate, with pyridoxal 5'-phosphate and leucine, with N-phosphopyridoxyl-leucine, and with N-phosphopyridoxyl-alpha-aminoadipate, at 2.67, 2.26, 1.75 and 1.67 A resolution, respectively. Enzyme contains a mobile alpha2-helix involved in substrate recognition | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminoadipate + pyruvate | - |
Thermus thermophilus | 2-oxoadipate + L-alanine | - |
? | |
2-oxoadipate + L-glutamate | - |
Thermus thermophilus | L-2-aminoadipate + 2-oxoglutarate | - |
? | |
L-leucine + 2-oxoglutarate | - |
Thermus thermophilus | 4-methyl-2-oxopentanoate + L-glutamate | - |
? | |
additional information | AAA-AT can recognize various kinds of substrates using the mobile alpha2 helix | Thermus thermophilus | ? | - |
? | |
phenylpyruvate + 2-aminoadipate | - |
Thermus thermophilus | L-phenylalanine + 2-oxoglutarate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AAA-AT | - |
Thermus thermophilus |
alpha-aminoadipate aminotransferase | - |
Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the crystal structures and site-directed mutagenesis reveales that intersubunit-electrostatic interactions contributes to the elevated thermostability of this enzyme | Thermus thermophilus |