Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activation only in the presence of pyridoxal phosphate | Rattus norvegicus | |
DTT | activation | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GSH | weak | Rattus norvegicus | |
L-cysteine | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rattus norvegicus | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
4 * 50000, reductive SDS-PAGE | Rattus norvegicus |
180000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.14 | - |
pH 8.8, 37°C | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, concentrated enzyme solution, in 5% polyethylene glycol plus 1 mg/ml pyridoxal 5'-phosphate, several months | Rattus norvegicus |
4°C, concentrated enzyme solution, several days | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-amino-2-methylpropanoate + 2-oxo-n-butanoate | - |
Rattus norvegicus | 2-methyl-3-oxopropanoate + 2-amino-n-butanoate | - |
? | |
(R)-3-amino-2-methylpropanoate + 2-oxo-n-pentanoate | - |
Rattus norvegicus | 2-methyl-3-oxopropanoate + norvaline | - |
? | |
(R)-3-amino-2-methylpropanoate + glyoxylate | - |
Rattus norvegicus | 2-methyl-3-oxopropanoate + glycine | - |
? | |
(R)-3-amino-2-methylpropanoate + oxaloacetate | - |
Rattus norvegicus | 2-methyl-3-oxopropanoate + L-aspartate | - |
? | |
(R)-3-amino-2-methylpropanoate + pyruvate | - |
Rattus norvegicus | 2-methyl-3-oxopropanoate + L-alanine | - |
? | |
5-aminolevulinic acid + pyruvate | - |
Rattus norvegicus | 5-oxolevulinic acid + L-alanine | - |
? | |
arginine + pyruvate | transamination at 40% the rate of (R)-3-amino-2-methylpropanoate | Rattus norvegicus | 2-oxo-5-guanidinovaleric acid + L-alanine | - |
? | |
beta-alanine + pyruvate | - |
Rattus norvegicus | malonic semialdehyde + L-alanine | - |
? | |
DL-3-amino-n-butanoate + pyruvate | - |
Rattus norvegicus | 3-oxobutanoate + L-alanine | - |
? | |
glycine + pyruvate | - |
Rattus norvegicus | glyoxylate + L-alanine | - |
? | |
additional information | overview | Rattus norvegicus | ? | - |
? | |
NG,NG'-dimethyl-(S)-arginine + pyruvate | - |
Rattus norvegicus | ? + L-alanine | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 50000, reductive SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | requirement, tightly bound | Rattus norvegicus |