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Literature summary for 2.6.1.42 extracted from

  • Taylor, R.T.; Jenkins, W.T.
    Leucine aminotransferase. II. Purification and characterization (1966), J. Biol. Chem., 241, 4396-4405.
    View publication on PubMed
Search for more literature for 2.6.1.42

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
 Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
hydroxylamine
-
 Sus scrofa
phenylhydrazine
-
 Sus scrofa
potassium cyanide
-
 Sus scrofa
Thiosemicarbazide
-
 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 sedimentation equilibrium Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-isoleucine + 2-oxoglutarate Sus scrofa
-
 3-methyl-2-oxopentanoate + L-glutamate
-
 r
L-leucine + 2-oxoglutarate Sus scrofa
-
 4-methyl-2-oxopentanoate + L-glutamate
-
 r
L-valine + 2-oxoglutarate Sus scrofa
-
 3-methyl-2-oxobutanoate + L-glutamate
-
 r

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
 pig, hog
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
heart heart muscle Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
50 53
-
 Sus scrofa

Storage Stability

Storage Stability Organism
-10°C, concentrated solution frozen for 1month retains 90% of her original activity Sus scrofa
5°C, pH 7.0, purified enzyme loses activity, about 20% in 1 week Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-2-aminoadipate + 2-oxoglutarate heart enzyme Sus scrofa 3-oxohexanedioic acid + L-glutamate
-
 r
DL-2-aminopimelate + 2-oxoglutarate heart enzyme Sus scrofa 2-oxoheptanedioate + L-glutamate
-
 r
DL-allo-isoleucine + 2-oxoglutarate heart enzyme Sus scrofa 3-methyl-2-oxopentanoate + L-glutamate
-
 r
L-2-aminobutyrate + 2-oxoglutarate heart enzyme Sus scrofa 2-oxobutanoate + L-glutamate
-
 r
L-isoleucine + 2-oxoglutarate
-
 Sus scrofa 3-methyl-2-oxopentanoate + L-glutamate
-
 r
L-leucine + 2-oxoglutarate
-
 Sus scrofa 4-methyl-2-oxopentanoate + L-glutamate
-
 r
L-methionine + 2-oxoglutarate
-
 Sus scrofa 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
 r
L-norleucine + 2-oxoglutarate
-
 Sus scrofa 2-oxohexanoate + L-glutamate
-
 r
L-norvaline + 2-oxoglutarate
-
 Sus scrofa 2-oxopentanoate + L-glutamate
-
 r
L-valine + 2-oxoglutarate
-
 Sus scrofa 3-methyl-2-oxobutanoate + L-glutamate
-
 r
additional information activity less than 3% with glycine, L-alanine, L-lysine, L-phenylalanine, L-tryptophan, D-alloisoleucine, D-valine, D-leucine, L-threonine, L-histidine, L-arginine, L-cysteine, DL-homocyteine, beta-aminoisobutyrate, gamma-aminobutyrate, alpha-aminoisobutyrate, DL-alpha-aminocaprylate, L-serine, DL-homoserine, DL-beta-aminobutyrate, DL-N-hydroxyleucine, DL-N-hydroxyvaline, L-aspartate, L-tyrosine, L-kynurenine, L-ornithine, DL-proline, L-glutamine, L-peniclliamine, DL-methionine sulfone, DL-methionine sulfoxide and DL-beta-hydroxy-leucine Sus scrofa ?
-
 ?
S-methyl-L-cysteine + 2-oxoglutarate heart enzyme Sus scrofa 3-(methylthio)-2-oxopropanoic acid + L-glutamate
-
 r

Synonyms

Synonyms Comment Organism
EC 2.6.1.6 related Sus scrofa
leucine transaminase
-
 Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3 8.5
-
 Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
 Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7 most stable in this range Sus scrofa

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Sus scrofa