Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Rattus norvegicus | |
2-mercaptoethanol | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.75 | - |
L-leucine | pH 8.2, 37°C | Rattus norvegicus | |
0.84 | - |
L-isoleucine | pH 8.2, 37°C | Rattus norvegicus | |
1 | - |
2-oxoglutarate | pH 8.2, 37°C | Rattus norvegicus | |
4.3 | - |
L-valine | pH 8.2, 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rattus norvegicus | 5737 | - |
cytoplasm | - |
Sus scrofa | 5737 | - |
microsome | - |
Rattus norvegicus | - |
- |
mitochondrion | - |
Sus scrofa | 5739 | - |
mitochondrion | mainly located in the cytoplasm | Rattus norvegicus | 5739 | - |
nucleus | - |
Rattus norvegicus | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate | Rattus norvegicus | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-isoleucine + 2-oxoglutarate | Sus scrofa | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | Sus scrofa | - |
4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | Rattus norvegicus | sole transaminase in fetal rat liver | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | Rattus norvegicus | - |
3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | Sus scrofa | - |
3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
additional information | Rattus norvegicus | specific for L-leucine, L-isoleucine or L-valine, no other amino acid would serve as an amino donor | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
rat, strain Wistar | - |
Sus scrofa | - |
pig, hog | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01855 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate | - |
Rattus norvegicus | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-isoleucine + 2-oxoglutarate | - |
Sus scrofa | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | - |
Rattus norvegicus | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | - |
Sus scrofa | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | sole transaminase in fetal rat liver | Rattus norvegicus | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | - |
Rattus norvegicus | 3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | - |
Sus scrofa | 3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
additional information | specific for L-leucine, L-isoleucine or L-valine, no other amino acid would serve as an amino donor | Rattus norvegicus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | Km 0.025 mM | Rattus norvegicus | |
pyridoxal 5'-phosphate | Km 0.0065 mM | Sus scrofa |