Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Neurospora crassa | 5829 | - |
mitochondrion | inner membrane-matrix | Neurospora crassa | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67500 | - |
mitochondrial isoenzyme, gel filtration | Neurospora crassa |
88000 | - |
cytoplasmic isoenzyme, gel filtration | Neurospora crassa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate | Neurospora crassa | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | Neurospora crassa | mitochondrial isoenzyme functions in the biosynthesis of L-isoleucine, L-leucine and L-valine, cytoplasmic isoenzyme functions in amino acid catabolism | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | Neurospora crassa | - |
3-methyl-2-oxobutanoate + L-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially | Neurospora crassa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25 | - |
cytoplasmic enzyme | Neurospora crassa |
48.8 | - |
mitochondrial enzyme | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + 2-oxoglutarate | mitochondrial enzyme | Neurospora crassa | 2-oxoglutarate + L-glutamate | - |
r | |
L-isoleucine + 2-oxoglutarate | - |
Neurospora crassa | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-isoleucine + 2-oxoglutarate | mitochondrial enzyme | Neurospora crassa | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | - |
Neurospora crassa | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | mitochondrial isoenzyme functions in the biosynthesis of L-isoleucine, L-leucine and L-valine, cytoplasmic isoenzyme functions in amino acid catabolism | Neurospora crassa | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-methionine + 2-oxoglutarate | cytoplasmic isoenzyme | Neurospora crassa | 4-methylsulfanyl-2-oxobutanoate + L-glutamate | - |
r | |
L-phenylalanine + 2-oxoglutarate | cytoplasmic isoenzyme | Neurospora crassa | phenylpyruvate + L-glutamate | - |
r | |
L-tyrosine + 2-oxoglutarate | cytoplasmic isoenzyme | Neurospora crassa | p-hydroxyphenylpyruvate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | - |
Neurospora crassa | 3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | mitochondrial enzyme | Neurospora crassa | 3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
additional information | purified mitochondrial enzyme is not active with L-phenylalanine | Neurospora crassa | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
cytoplasmic enzyme | Neurospora crassa |
8.8 | - |
mitochondrial enzyme | Neurospora crassa |