Cloned (Comment) | Organism |
---|---|
K-12 strain carries the ilvE gene both on the host chromosome and on a plasmid | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
DL-2-oxo-3-methyl-n-pentanoate | pH 8.0, 37°C | Escherichia coli | |
0.52 | - |
L-isoleucine | pH 8.0, 37°C | Escherichia coli | |
0.56 | - |
2-Oxoisohexanoate | pH 8.0, 37°C | Escherichia coli | |
0.56 | - |
2-oxoisopentanoate | pH 8.0, 37°C | Escherichia coli | |
0.58 | - |
L-leucine | pH 8.0, 37°C | Escherichia coli | |
1.28 | - |
2-oxoglutarate | pH 8.0, 37°C | Escherichia coli | |
3.13 | - |
L-valine | pH 8.0, 37°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31500 | - |
6 * 31500, SDS-PAGE | Escherichia coli |
182300 | - |
sedimentation equilibrium centrifugation | Escherichia coli |
185000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate | Escherichia coli | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | Escherichia coli | - |
4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | Escherichia coli | - |
3-methyl-2-oxobutanoate + L-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K-12 | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.9 | - |
substrate L-valine + 2-oxoglutarate | Escherichia coli |
27.3 | - |
substrate L-isoleucine + 2-oxoglutarate | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate | - |
Escherichia coli | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | - |
Escherichia coli | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-phenylalanine + 2-oxoglutarate | - |
Escherichia coli | phenylpyruvate + L-glutamate | - |
r | |
L-tyrosine + 2-oxoglutarate | - |
Escherichia coli | p-hydroxyphenylpyruvate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | - |
Escherichia coli | 3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
additional information | purified enzyme has no measurable L-aspartate-2-oxoglutarate activity | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 31500, SDS-PAGE | Escherichia coli |