Cloned (Comment) | Organism |
---|---|
ilvGEDAY gene cluster expressed in Escherichia coli JA199 | Salmonella enterica subsp. enterica serovar Typhimurium |
Crystallization (Comment) | Organism |
---|---|
crystallized from recombinant Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33920 | - |
6 * 33920, calculated from amino acid sequence | Salmonella enterica subsp. enterica serovar Typhimurium |
204900 | - |
calculated from amino acid sequence | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate | Salmonella enterica subsp. enterica serovar Typhimurium | biosynthesis of L-isoleucine | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | Salmonella enterica subsp. enterica serovar Typhimurium | biosynthesis of L-leucine | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | Salmonella enterica subsp. enterica serovar Typhimurium | biosynthesis of L-valine | 3-methyl-2-oxobutanoate + L-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate | biosynthesis of L-isoleucine | Salmonella enterica subsp. enterica serovar Typhimurium | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | biosynthesis of L-leucine | Salmonella enterica subsp. enterica serovar Typhimurium | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | biosynthesis of L-valine | Salmonella enterica subsp. enterica serovar Typhimurium | 3-methyl-2-oxobutanoate + L-glutamate | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 33920, calculated from amino acid sequence | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium |