Cloned (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
carboxymethoxylamine | mixed-type inhibition, acts also as growth inhibitor of organism | Mycobacterium tuberculosis | |
O-(t-butyl)hydroxylamine | mixed-type inhibition, acts also as growth inhibitor of organism | Mycobacterium tuberculosis | |
O-allylhydroxylamine | mixed-type inhibition, acts also as growth inhibitor of organism | Mycobacterium tuberculosis | |
O-Benzylhydroxylamine | mixed-type inhibition, acts also as growth inhibitor of organism | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.77 | - |
L-valine | cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.5 | - |
L-leucine | cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.85 | - |
L-isoleucine | cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C | Mycobacterium tuberculosis | |
4.2 | - |
4-methylthio-2-oxo-butanoate | cosubstrate leucine, pH 7.4, 37°C | Mycobacterium tuberculosis | |
5.79 | - |
L-valine | cosubstrate 2-oxoglutarate, pH 7.4, 37°C | Mycobacterium tuberculosis | |
6.02 | - |
L-leucine | cosubstrate 2-oxoglutarate, pH 7.4, 37°C | Mycobacterium tuberculosis | |
6.16 | - |
L-isoleucine | cosubstrate 2-oxoglutarate, pH 7.4, 37°C | Mycobacterium tuberculosis | |
6.95 | - |
2-oxoglutarate | cosubstrate L-leucine, pH 7.4, 37°C | Mycobacterium tuberculosis | |
7.44 | - |
L-phenylalanine | cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C | Mycobacterium tuberculosis | |
9.53 | - |
L-glutamate | cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.17 | - |
substrate L-phenylalanine, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C | Mycobacterium tuberculosis |
2.58 | - |
substrate L-valine, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C | Mycobacterium tuberculosis |
3.65 | - |
substrate L-leucine, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C | Mycobacterium tuberculosis |
4.22 | - |
substrate 4-methylthio-2-oxo-butanoate, cosubstrate leucine, pH 7.4, 37°C | Mycobacterium tuberculosis |
4.28 | - |
substrate L-isoleucine, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C | Mycobacterium tuberculosis |
5.7 | - |
substrate L-glutamate, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C | Mycobacterium tuberculosis |
11.82 | - |
substrate L-valine, cosubstrate 2-oxoglutarate, pH 7.4, 37°C | Mycobacterium tuberculosis |
12.8 | - |
substrate 2-oxoglutarate, cosubstrate L-leucine, pH 7.4, 37°C | Mycobacterium tuberculosis |
13.44 | - |
substrate L-leucine, cosubstrate 2-oxoglutarate, pH 7.4, 37°C | Mycobacterium tuberculosis |
14.35 | - |
substrate L-isoleucine, cosubstrate 2-oxoglutarate, pH 7.4, 37°C | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + 4-methylthio-2-oxo-butanoate | - |
Mycobacterium tuberculosis | 2-oxoglutarate + L-methionine | - |
? | |
L-glutamate + 4-methylthio-2-oxo-butanoate | - |
Mycobacterium tuberculosis H37Rv | 2-oxoglutarate + L-methionine | - |
? | |
L-isoleucine + 2-oxoglutarate | - |
Mycobacterium tuberculosis | 3-methyl-2-oxopentanoate + L-glutamate | - |
? | |
L-isoleucine + 2-oxoglutarate | - |
Mycobacterium tuberculosis H37Rv | 3-methyl-2-oxopentanoate + L-glutamate | - |
? | |
L-isoleucine + 4-methylthio-2-oxo-butanoate | - |
Mycobacterium tuberculosis | 3-methyl-2-oxopentanoate + L-methionine | - |
? | |
L-isoleucine + 4-methylthio-2-oxo-butanoate | - |
Mycobacterium tuberculosis H37Rv | 3-methyl-2-oxopentanoate + L-methionine | - |
? | |
L-leucine + 4-methylthio-2-oxo-butanoate | - |
Mycobacterium tuberculosis | 4-methyl-2-oxopentanoate + L-methionine | - |
? | |
L-leucine + 4-methylthio-2-oxo-butanoate | - |
Mycobacterium tuberculosis H37Rv | 4-methyl-2-oxopentanoate + L-methionine | - |
? | |
L-phenylalanine + 4-methylthio-2-oxo-butanoate | - |
Mycobacterium tuberculosis | phenylpyruvate + L-methionine | - |
? | |
L-phenylalanine + 4-methylthio-2-oxo-butanoate | - |
Mycobacterium tuberculosis H37Rv | phenylpyruvate + L-methionine | - |
? | |
L-valine + 4-methylthio-2-oxo-butanoate | - |
Mycobacterium tuberculosis | 2-oxoisopentanoate + L-methionine | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0082 | - |
O-Benzylhydroxylamine | competitive component of mixed-type inhibition, pH 7.4, 37°C | Mycobacterium tuberculosis | |
0.011 | - |
O-(t-butyl)hydroxylamine | competitive component of mixed-type inhibition, pH 7.4, 37°C | Mycobacterium tuberculosis | |
0.01424 | - |
carboxymethoxylamine | uncompetitive component of mixed-type inhibition, pH 7.4, 37°C | Mycobacterium tuberculosis | |
0.0209 | - |
carboxymethoxylamine | competitive component of mixed-type inhibition, pH 7.4, 37°C | Mycobacterium tuberculosis | |
0.0216 | - |
O-allylhydroxylamine | competitive component of mixed-type inhibition, pH 7.4, 37°C | Mycobacterium tuberculosis | |
0.0841 | - |
O-Benzylhydroxylamine | uncompetitive component of mixed-type inhibition, pH 7.4, 37°C | Mycobacterium tuberculosis | |
0.0856 | - |
O-(t-butyl)hydroxylamine | uncompetitive component of mixed-type inhibition, pH 7.4, 37°C | Mycobacterium tuberculosis | |
0.2 | - |
O-allylhydroxylamine | or higher, uncompetitive component of mixed-type inhibition, pH 7.4, 37°C | Mycobacterium tuberculosis |