Literature summary for 2.6.1.42 extracted from

Venos, E.S.; Knodel, M.H.; Radford, C.L.; Berger, B.J.
Branched-chain amino acid aminotransferase and methionine formation in Mycobacterium tuberculosis (2004), BMC Microbiol., 4, 39.

Search for more literature for 2.6.1.42

Cloned(Commentary)

Cloned (Comment)	Organism
-	Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
carboxymethoxylamine	mixed-type inhibition, acts also as growth inhibitor of organism	Mycobacterium tuberculosis
O-(t-butyl)hydroxylamine	mixed-type inhibition, acts also as growth inhibitor of organism	Mycobacterium tuberculosis
O-allylhydroxylamine	mixed-type inhibition, acts also as growth inhibitor of organism	Mycobacterium tuberculosis
O-Benzylhydroxylamine	mixed-type inhibition, acts also as growth inhibitor of organism	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.77	-	L-valine	cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C	Mycobacterium tuberculosis
2.5	-	L-leucine	cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C	Mycobacterium tuberculosis
2.85	-	L-isoleucine	cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C	Mycobacterium tuberculosis
4.2	-	4-methylthio-2-oxo-butanoate	cosubstrate leucine, pH 7.4, 37°C	Mycobacterium tuberculosis
5.79	-	L-valine	cosubstrate 2-oxoglutarate, pH 7.4, 37°C	Mycobacterium tuberculosis
6.02	-	L-leucine	cosubstrate 2-oxoglutarate, pH 7.4, 37°C	Mycobacterium tuberculosis
6.16	-	L-isoleucine	cosubstrate 2-oxoglutarate, pH 7.4, 37°C	Mycobacterium tuberculosis
6.95	-	2-oxoglutarate	cosubstrate L-leucine, pH 7.4, 37°C	Mycobacterium tuberculosis
7.44	-	L-phenylalanine	cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C	Mycobacterium tuberculosis
9.53	-	L-glutamate	cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.17	-	substrate L-phenylalanine, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C	Mycobacterium tuberculosis
2.58	-	substrate L-valine, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C	Mycobacterium tuberculosis
3.65	-	substrate L-leucine, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C	Mycobacterium tuberculosis
4.22	-	substrate 4-methylthio-2-oxo-butanoate, cosubstrate leucine, pH 7.4, 37°C	Mycobacterium tuberculosis
4.28	-	substrate L-isoleucine, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C	Mycobacterium tuberculosis
5.7	-	substrate L-glutamate, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C	Mycobacterium tuberculosis
11.82	-	substrate L-valine, cosubstrate 2-oxoglutarate, pH 7.4, 37°C	Mycobacterium tuberculosis
12.8	-	substrate 2-oxoglutarate, cosubstrate L-leucine, pH 7.4, 37°C	Mycobacterium tuberculosis
13.44	-	substrate L-leucine, cosubstrate 2-oxoglutarate, pH 7.4, 37°C	Mycobacterium tuberculosis
14.35	-	substrate L-isoleucine, cosubstrate 2-oxoglutarate, pH 7.4, 37°C	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamate + 4-methylthio-2-oxo-butanoate	-	Mycobacterium tuberculosis	2-oxoglutarate + L-methionine	-	?
L-glutamate + 4-methylthio-2-oxo-butanoate	-	Mycobacterium tuberculosis H37Rv	2-oxoglutarate + L-methionine	-	?
L-isoleucine + 2-oxoglutarate	-	Mycobacterium tuberculosis	3-methyl-2-oxopentanoate + L-glutamate	-	?
L-isoleucine + 2-oxoglutarate	-	Mycobacterium tuberculosis H37Rv	3-methyl-2-oxopentanoate + L-glutamate	-	?
L-isoleucine + 4-methylthio-2-oxo-butanoate	-	Mycobacterium tuberculosis	3-methyl-2-oxopentanoate + L-methionine	-	?
L-isoleucine + 4-methylthio-2-oxo-butanoate	-	Mycobacterium tuberculosis H37Rv	3-methyl-2-oxopentanoate + L-methionine	-	?
L-leucine + 4-methylthio-2-oxo-butanoate	-	Mycobacterium tuberculosis	4-methyl-2-oxopentanoate + L-methionine	-	?
L-leucine + 4-methylthio-2-oxo-butanoate	-	Mycobacterium tuberculosis H37Rv	4-methyl-2-oxopentanoate + L-methionine	-	?
L-phenylalanine + 4-methylthio-2-oxo-butanoate	-	Mycobacterium tuberculosis	phenylpyruvate + L-methionine	-	?
L-phenylalanine + 4-methylthio-2-oxo-butanoate	-	Mycobacterium tuberculosis H37Rv	phenylpyruvate + L-methionine	-	?
L-valine + 4-methylthio-2-oxo-butanoate	-	Mycobacterium tuberculosis	2-oxoisopentanoate + L-methionine	-	?

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0082	-	O-Benzylhydroxylamine	competitive component of mixed-type inhibition, pH 7.4, 37°C	Mycobacterium tuberculosis
0.011	-	O-(t-butyl)hydroxylamine	competitive component of mixed-type inhibition, pH 7.4, 37°C	Mycobacterium tuberculosis
0.01424	-	carboxymethoxylamine	uncompetitive component of mixed-type inhibition, pH 7.4, 37°C	Mycobacterium tuberculosis
0.0209	-	carboxymethoxylamine	competitive component of mixed-type inhibition, pH 7.4, 37°C	Mycobacterium tuberculosis
0.0216	-	O-allylhydroxylamine	competitive component of mixed-type inhibition, pH 7.4, 37°C	Mycobacterium tuberculosis
0.0841	-	O-Benzylhydroxylamine	uncompetitive component of mixed-type inhibition, pH 7.4, 37°C	Mycobacterium tuberculosis
0.0856	-	O-(t-butyl)hydroxylamine	uncompetitive component of mixed-type inhibition, pH 7.4, 37°C	Mycobacterium tuberculosis
0.2	-	O-allylhydroxylamine	or higher, uncompetitive component of mixed-type inhibition, pH 7.4, 37°C	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)