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Literature summary for 2.6.1.42 extracted from
- Purification, crystallization and preliminary X-ray crystallographic analysis of branched-chain aminotransferase from Deinococcus radiodurans (2007), Acta Crystallogr. Sect. F, 63, 492-494.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain JM109 | Deinococcus radiodurans |
| expression in Escherichia coli | Deinococcus radiodurans |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| diffraction to 2.5 A resolution, space group P212121 | Deinococcus radiodurans |
| hanging drop vapour diffusion method using 20% (w/v) PEG 8000 and 0.2 M MgCl2 in 0.1 M Tris buffer pH 8.5 | Deinococcus radiodurans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40890 | - |
ESI-tandem mass spectrometry | Deinococcus radiodurans |
| 40891 | - |
x * 41000, SDS-PAGE, x * 40891, calculated | Deinococcus radiodurans |
| 40900 | - |
SDS-PAGE | Deinococcus radiodurans |
| 41000 | - |
x * 41000, SDS-PAGE, x * 40891, calculated | Deinococcus radiodurans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Deinococcus radiodurans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-tag Ni2+-NTA column chromatography | Deinococcus radiodurans |
| recombinant protein | Deinococcus radiodurans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-isoleucine + 2-oxoglutarate | - |
Deinococcus radiodurans | 3-methyl-2-oxopentanoate + L-glutamate | - |
? |  |
| L-leucine + 2-oxoglutarate | - |
Deinococcus radiodurans | 4-methyl-2-oxopentanoate + L-glutamate | - |
? | |
| L-valine + 2-oxoglutarate | - |
Deinococcus radiodurans | 3-methyl-2-oxobutanoate + L-glutamate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 41000, SDS-PAGE, x * 40891, calculated | Deinococcus radiodurans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BcaT | - |
Deinococcus radiodurans |
| branched-chain amino-acid aminotransferase | - |
Deinococcus radiodurans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | required | Deinococcus radiodurans | |
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Release 2023.1 (January 2023)
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