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Literature summary for 2.6.1.42 extracted from
- Crystal structures of complexes of the branched-chain aminotransferase from Deinococcus radiodurans with alpha-ketoisocaproate and L-glutamate suggest the radiation resistance of this enzyme for catalysis (2012), J. Bacteriol., 194, 6206-6216.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| His-tagged protein expressed in Escherichia coli JM109 | Deinococcus radiodurans |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging-drop vapor diffusion method, PEG 8000, pH 7.4, 20°C | Deinococcus radiodurans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K202R | active site residue, no activity detected | Deinococcus radiodurans |
| R145E | no activity detected | Deinococcus radiodurans |
| Y71A/R145A | no activity detected | Deinococcus radiodurans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Deinococcus radiodurans | Q9RTX5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immobilized metal ion affinity chromatography (Ni2+) | Deinococcus radiodurans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
62.5% activity with 2-oxoisovalerate, 34.9% activity with 3-methyl-2-oxovalerate, 22.7% activity with 2-oxo-4-methylthiobutyrate, 57.1% activity with 2-oxohexanoate, 51.0% activity with 2-oxovalerate | Deinococcus radiodurans |
| 180 | - |
substrates 2-oxoisocaproate and L-glutamate, pH 8.3, 25°C | Deinococcus radiodurans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-isoleucine + 2-oxoglutarate | - |
Deinococcus radiodurans | 3-methyl-2-oxopentanoic acid + L-glutamate | - |
r |  |
| L-leucine + 2-oxoglutarate | - |
Deinococcus radiodurans | 4-methyl-2-oxopentanoic acid + L-glutamate | - |
r | |
| L-valine + 2-oxoglutarate | - |
Deinococcus radiodurans | 3-methyl-2-oxobutanoic acid + L-glutamate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | crystal structure | Deinococcus radiodurans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| branched-chain aminotransferase | - |
Deinococcus radiodurans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable at least for 10 min, about 40% activity after 10 min at 65% | Deinococcus radiodurans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Deinococcus radiodurans | |
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Release 2023.1 (January 2023)
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