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Literature summary for 2.6.1.42 extracted from

  • Boyko, K.M.; Stekhanova, T.N.; Nikolaeva, A.Y.; Mardanov, A.V.; Rakitin, A.L.; Ravin, N.V.; Bezsudnova, E.Y.; Popov, V.O.
    First structure of archaeal branched-chain amino acid aminotransferase from Thermoproteus uzoniensis specific for L-amino acids and R-amines (2016), Extremophiles, 20, 215-225.
    View publication on PubMed
Search for more literature for 2.6.1.42

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermoproteus uzoniensis

Crystallization (Commentary)

Crystallization (Comment) Organism
to 2.0 A resolution, three protein subunit per asymmetric unit. The subunit has a pyridoxal phosphate type IV fold and consisits of a small domain, a larege domain and an interdomain loop Thermoproteus uzoniensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32800
-
 2 * 32800, calculated Thermoproteus uzoniensis
60000
-
 gel filtration Thermoproteus uzoniensis

Organism

Organism UniProt Comment Textmining
Thermoproteus uzoniensis F2L0W0
-
-
Thermoproteus uzoniensis 768-20 F2L0W0
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.77
-
 substrate L-alanine, pH 8.0, 65°C Thermoproteus uzoniensis
1
-
 substrate L-isoleucine, pH 8.0, 65°C Thermoproteus uzoniensis
1.7
-
 substrate L-leucine, pH 8.0, 65°C Thermoproteus uzoniensis
3.6
-
 substrate L-valine, pH 8.0, 65°C Thermoproteus uzoniensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-alpha-methylbenzylamine + pyruvate small but significant activity, the (S)-enantiomer is not a substrate Thermoproteus uzoniensis alpha-methylbenzaldehyde + L-alanine
-
 ?
(R)-alpha-methylbenzylamine + pyruvate small but significant activity, the (S)-enantiomer is not a substrate Thermoproteus uzoniensis 768-20 alpha-methylbenzaldehyde + L-alanine
-
 ?
L-alanine + 4-methyl-2-oxopentanoate no substrate: D-alanine Thermoproteus uzoniensis pyruvate + L-leucine
-
 ?
L-alanine + 4-methyl-2-oxopentanoate no substrate: D-alanine Thermoproteus uzoniensis 768-20 pyruvate + L-leucine
-
 ?
L-isoleucine + pyruvate
-
 Thermoproteus uzoniensis 2-oxoisohexanoate + L-alanine
-
 ?
L-isoleucine + pyruvate
-
 Thermoproteus uzoniensis 768-20 2-oxoisohexanoate + L-alanine
-
 ?
L-leucine + pyruvate
-
 Thermoproteus uzoniensis 4-methyl-2-oxopentanoate + L-alanine
-
 ?
L-leucine + pyruvate
-
 Thermoproteus uzoniensis 768-20 4-methyl-2-oxopentanoate + L-alanine
-
 ?
L-valine + pyruvate
-
 Thermoproteus uzoniensis 3-methyl-2-oxobutanoate + L-alanine
-
 ?
L-valine + pyruvate
-
 Thermoproteus uzoniensis 768-20 3-methyl-2-oxobutanoate + L-alanine
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 32800, calculated Thermoproteus uzoniensis

Synonyms

Synonyms Comment Organism
TUZN1299
-
 Thermoproteus uzoniensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
 reaction rate increases with increasing temperature up to 95°C Thermoproteus uzoniensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 5 h, no loss of activity, 24 h, about 65% residual activity Thermoproteus uzoniensis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate covalently bound to conserved residue Lys150, crystallization data Thermoproteus uzoniensis