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Literature summary for 2.6.1.43 extracted from
- Characteristics of L-alanine:4,5-dioxovaleric acid transaminase: an alternate pathway of heme biosynthesis in yeast (1990), Arch. Biochem. Biophys., 277, 122-129.
General Stability
| General Stability | Organism |
|---|---|
| repeated freezing and thawing inactivates the enzyme, stability of the enzyme is enhanced by presence of 5% glycerol | Candida albicans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | EDTA, N-ethylmaleimide. iodoacetic acid, cysteine and beta-mercaptoethanol are no inhibitors | Candida albicans | |
| p-chloromercuribenzoate | - |
Candida albicans |  |
| Tris-HCl | - |
Candida albicans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00028 | - |
L-alanine | pH 7.2, 60°C | Candida albicans | |
| 0.0105 | - |
4,5-Dioxopentanoate | pH 7.2, 60°C | Candida albicans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Candida albicans | 5829 | - |
| mitochondrion | - |
Candida albicans | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57544 | - |
1 * 57544, SDS-PAGE | Candida albicans |
| 59000 | - |
gel filtration | Saccharomyces cerevisiae |
| 59000 | - |
gel filtration | Candida albicans |
| 60260 | - |
gel filtration | Candida albicans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-alanine + 4,5-dioxopentanoate | Saccharomyces cerevisiae | - |
5-aminolevulinate + pyruvate | - |
ir | |
| L-alanine + 4,5-dioxopentanoate | Candida albicans | - |
5-aminolevulinate + pyruvate | - |
ir | |
| L-alanine + 4,5-dioxopentanoate | Candida albicans 3100 | - |
5-aminolevulinate + pyruvate | - |
ir | |
| L-alanine + 4,5-dioxopentanoate | Saccharomyces cerevisiae 3059 S288C | - |
5-aminolevulinate + pyruvate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | - |
3100 | - |
| Candida albicans 3100 | - |
3100 | - |
| Saccharomyces cerevisiae | - |
3059, S288C | - |
| Saccharomyces cerevisiae 3059 S288C | - |
3059, S288C | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
- |
Candida albicans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.186 | - |
- |
Candida albicans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stored in ice for 3 months without any appreciable loss of enzyme activity | Candida albicans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycine + 4,5-dioxopentanoate | - |
Candida albicans | 5-aminolevulinate + glyoxylate | - |
ir | |
| glycine + 4,5-dioxopentanoate | - |
Candida albicans 3100 | 5-aminolevulinate + glyoxylate | - |
ir | |
| L-alanine + 4,5-dioxopentanoate | - |
Saccharomyces cerevisiae | 5-aminolevulinate + pyruvate | - |
ir | |
| L-alanine + 4,5-dioxopentanoate | - |
Candida albicans | 5-aminolevulinate + pyruvate | - |
ir | |
| L-alanine + 4,5-dioxopentanoate | - |
Candida albicans 3100 | 5-aminolevulinate + pyruvate | - |
ir | |
| L-alanine + 4,5-dioxopentanoate | - |
Saccharomyces cerevisiae 3059 S288C | 5-aminolevulinate + pyruvate | - |
ir | |
| L-arginine + 4,5-dioxopentanoate | 6.8% of the activity with L-alanine | Candida albicans | 5-aminolevulinate + 5-guanidino-2-oxopentanoate | - |
ir | |
| L-asparagine + 4,5-dioxopentanoate | 74% of the activity with L-alanine | Candida albicans | 5-aminolevulinate + 4-amino-2,4-dioxobutanoic acid | - |
ir | |
| L-aspartate + 4,5-dioxopentanoate | 39% of the activity with L-alanine | Candida albicans | 5-aminolevulinate + oxaloacetate | - |
ir | |
| L-glutamate + 4,5-dioxopentanoate | 39% of the activity with L-alanine | Candida albicans | 5-aminolevulinate + 2-oxoglutarate | - |
ir | |
| L-glutamate + 4,5-dioxopentanoate | 39% of the activity with L-alanine | Candida albicans 3100 | 5-aminolevulinate + 2-oxoglutarate | - |
ir | |
| L-glutamine + 4,5-dioxopentanoate | 74% of the activity with L-alanine | Candida albicans | 5-aminolevulinate + 2-oxoglutaramate | - |
? | |
| additional information | cannot utilize 2-oxoglutarate nor pyruvate as amino acceptors | Candida albicans | ? | - |
? | |
| additional information | cannot utilize 2-oxoglutarate nor pyruvate as amino acceptors | Candida albicans 3100 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 59000, SDS-PAGE | Saccharomyces cerevisiae |
| monomer | 1 * 57544, SDS-PAGE | Candida albicans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Saccharomyces cerevisiae |
| 60 | - |
- |
Candida albicans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Candida albicans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Saccharomyces cerevisiae | |
| pyridoxal 5'-phosphate | Km 0.0005 mM | Candida albicans | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Candida albicans | isoelectric focusing using Phast GEl IEF 3-9 | 4.8 | 4.7 |
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