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Literature summary for 2.6.1.44 extracted from

  • Okuno, E.; Minatogawa, Y.; Kido, R.
    Co-purification of alanine-glyoxylate aminotransferase with 2-aminobutyrate aminotransferase in rat kidney (1982), Biochim. Biophys. Acta, 715, 97-104.
    View publication on PubMed
Search for more literature for 2.6.1.44

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
 glyoxylate pH 8.0, 37°C, alanine as amino donor Rattus norvegicus
0.67
-
 glyoxylate pH 8.0, 37°C, 2-aminobutyrate as amino donor Rattus norvegicus
2.5
-
 pyruvate pH 8.0, 37°C, 2-aminobutyrate as amino donor Rattus norvegicus
2.7
-
 2-oxobutyrate pH 8.0, 37°C, alanine as amino donor Rattus norvegicus
2.9
-
 L-alanine pH 8.0, 37°C, 2-oxobutyrate as amino acceptor Rattus norvegicus
11
-
 L-alanine pH 8.0, 37°C, glyoxylate as amino acceptor Rattus norvegicus
60
-
 2-aminobutyrate pH 8.0, 37°C, glyoxylate as amino acceptor Rattus norvegicus
100
-
 2-aminobutyrate pH 8.0, 37°C, pyruvate as amino acceptor Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 4 * 56000, SDS-PAGE Rattus norvegicus
175000
-
 liver isoenzyme 2, gel filtration Rattus norvegicus
213000
-
 analytical ultracentrifugation Rattus norvegicus
220000
-
 gel filtration Rattus norvegicus
236000
-
 sucrose density gradient centrifugation Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine + glyoxylate Rattus norvegicus
-
 pyruvate + glycine
-
 ir

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
co-purified with 2-aminobutyrate aminotranferase Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Rattus norvegicus
-
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
160
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, crude homogenate can be stored for 6-12 months with little loss of activity Rattus norvegicus
-20°C, purified enzyme, 50% glycerol, can be stored for at least 3 months with little loss in activity Rattus norvegicus
-20°C, purified enzyme, freezing results in formation of floculence and complete loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-aminobutyrate + glyoxylate
-
 Rattus norvegicus 2-oxobutanoate + glycine
-
 ir
L-alanine + 2-oxobutyrate
-
 Rattus norvegicus pyruvate + 2-aminobutanoate
-
 ir
L-alanine + 4-methylthio-2-oxobutyrate poor amino acceptor Rattus norvegicus pyruvate + L-methionine
-
 ir
L-alanine + glyoxylate
-
 Rattus norvegicus pyruvate + glycine
-
 ir
L-alanine + pyruvate
-
 Rattus norvegicus pyruvate + L-alanine
-
 ir
L-aspartate + glyoxylate
-
 Rattus norvegicus 2-oxosuccinate + glycine
-
 ir
L-serine + glyoxylate
-
 Rattus norvegicus 3-hydroxy-2-oxopropanoate + glycine
-
 ir
additional information 2-oxoglutarate is not an amino acceptor, little or no activity with asparagine, glutamine, glutamate, threonine, cysteine, methionine, arginine, leucine, valine, isoleucine, phenylalanine, tryptophan, histidine or tyrosine as amino donors Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 56000, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
alanine-glyoxylate aminotransferase isoenzyme 2
-
 Rattus norvegicus
EC 2.6.1.51 serine-pyruvate aminotransferase identical with liver isoenzyme 1 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate Km 0.02 mM Rattus norvegicus