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Literature summary for 2.6.1.44 extracted from

  • Takada, Y.; Noguchi, T.
    Characteristics of alanine: glyoxylate aminotransferase from Saccharomyces cerevisiae, a regulatory enzyme in the glyoxylate pathway of glycine and serine biosynthesis from tricarboxylic acid-cycle intermediates (1985), Biochem. J., 231, 157-163.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
amino-oxyacetic acid
-
Saccharomyces cerevisiae
hydroxylamine
-
Saccharomyces cerevisiae
Isonicotinic acid hydrazide
-
Saccharomyces cerevisiae
Semicarbazide
-
Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
glyoxylate pH 8.2, 37°C, isoenzyme 2, alanine as amino donor Saccharomyces cerevisiae
2.1
-
L-alanine pH 8.2, 37°C, glyoxylate as amino acceptor Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
2 * 40000, SDS-PAGE Saccharomyces cerevisiae
80000
-
sucrose-density-gradient centrifugation Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine + glyoxylate Saccharomyces cerevisiae regulatory enzyme in the glyoxylate pathway of glycine and serine biosynthesis from tricarboxylic acid-cycle intermediates pyruvate + glycine
-
ir

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
101D, commercial baker's yeast
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
180.2
-
-
Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-20°C, 25 mM-potassium phosphate buffer, pH 7.5, containing 100 mM NaCl, 0.1 mM pyridoxal 5'-phosphate and 10% glycerol, my be stored for at least 3 months without loss of activity Saccharomyces cerevisiae
0-4°C, little or no activity is lost when stored for 2 weeks Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + glyoxylate
-
Saccharomyces cerevisiae pyruvate + glycine
-
ir
L-alanine + glyoxylate regulatory enzyme in the glyoxylate pathway of glycine and serine biosynthesis from tricarboxylic acid-cycle intermediates Saccharomyces cerevisiae pyruvate + glycine
-
ir
additional information utilizes only glyoxylate as amino acceptor, hydroxypyruvate, 2-oxoglutarate, phenylpyruvate and 2-oxo-4-methyl-thiobutyrate are inactive, only L-alanine as amino donor, L-serine, L-threonine, L-glutamic acid, L-glutamine, L-aspartic acid, L-asparagine, L-ornithine, L-leucine, L-valine, L-isoleucine, L-histidine, L-phenylalanine, L-tryptophan and L-tyrosine are no substrates Saccharomyces cerevisiae ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Saccharomyces cerevisiae

pI Value

Organism Comment pI Value Maximum pI Value
Saccharomyces cerevisiae isoelectric focusing of the crude extract on a pH 3.0-10 pharmalyte gradient, 3 activity peaks with pI 7.4, 7.8 and 8.3
-
7