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Literature summary for 2.6.1.44 extracted from

  • Sakuraba, H.; Fujiwara, S.; Noguchi, T.
    Purification and characterization of peroxisomal apo and holo alanine:glyoxylate aminotransferase from bird liver (1991), Arch. Biochem. Biophys., 286, 453-460.
    View publication on PubMed

General Stability

General Stability Organism
pyridoxal 5'-phosphate stabilizes during purification Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion apoenzyme in the peroxisomes, holoenzyme in the mitochondrion Gallus gallus 5739
-
peroxisome apoenzyme in the peroxisomes, holoenzyme in the mitochondrion Gallus gallus 5777
-
peroxisome apoenzyme in the peroxisomes, holoenzyme in the mitochondrion Phasianus versicolor 5777
-
peroxisome apoenzyme in the peroxisomes, holoenzyme in the mitochondrion Uroloncha striata domestica 5777
-
peroxisome holoenzyme in the peroxisomes Columba livia 5777
-
peroxisome holoenzyme in the peroxisomes Anas platyrhynchos domestica 5777
-
peroxisome holoenzyme in the peroxisomes Anser anser domesticus 5777
-
peroxisome holoenzyme in the peroxisomes Melopsittacus undulatus 5777
-
peroxisome holoenzyme in the peroxisomes Passer montanus saturatus 5777
-
peroxisome holoenzyme in the peroxisomes Padda oryzivora 5777
-
peroxisome holoenzyme in the peroxisomes Serinus canaria 5777
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
1 * 45000, apoenzyme, SDS-PAGE Gallus gallus
45000
-
2 * 45000, holoenzyme, SDS-PAGE Columba livia
50000
-
4 * 50000, mitochondrial holoenzyme, antibody cross reaction Gallus gallus
50000
-
4 * 50000, mitochondrial holoenzyme, antibody cross reaction Columba livia
90000
-
gel filtration, sucrose density gradient centrifugation Columba livia
200000
-
mitochondrial holo enzyme, sucrose density gradient centrifugation Gallus gallus
200000
-
mitochondrial holo enzyme, sucrose density gradient centrifugation Columba livia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine + glyoxylate Gallus gallus
-
pyruvate + glycine
-
ir
L-alanine + glyoxylate Columba livia
-
pyruvate + glycine
-
ir

Organism

Organism UniProt Comment Textmining
Anas platyrhynchos domestica
-
duck
-
Anser anser domesticus
-
goose
-
Columba livia
-
pigeon
-
Gallus gallus
-
white leghorn, bantam
-
Melopsittacus undulatus
-
australian bugerigar
-
Padda oryzivora
-
Java sparrow
-
Passer montanus saturatus
-
sparrow
-
Phasianus versicolor
-
pheasant
-
Serinus canaria
-
canary
-
Uroloncha striata domestica
-
japanese mannikin
-

Purification (Commentary)

Purification (Comment) Organism
-
Gallus gallus
-
Columba livia

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
Gallus gallus
-
liver
-
Gallus gallus
-
liver
-
Columba livia
-
liver
-
Anas platyrhynchos domestica
-
liver
-
Anser anser domesticus
-
liver
-
Melopsittacus undulatus
-
liver
-
Passer montanus saturatus
-
liver
-
Phasianus versicolor
-
liver
-
Padda oryzivora
-
liver
-
Serinus canaria
-
liver
-
Uroloncha striata domestica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.6
-
-
Columba livia

Storage Stability

Storage Stability Organism
-20°C, peroxisomal apoenzyme, may be stored for at least 4 weeks without loss of activity Gallus gallus
-20°C, peroxisomal holoenzyme, may be stored for at least 4 weeks without loss of activity Columba livia
4°C, peroxisomal apoenzyme, 50% activity is lost when stored for 6 days Gallus gallus
4°C, peroxisomal holoenzyme, 50% activity is lost when stored for 6 days Columba livia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + glyoxylate
-
Gallus gallus pyruvate + glycine
-
ir
L-alanine + glyoxylate
-
Columba livia pyruvate + glycine
-
ir

Subunits

Subunits Comment Organism
dimer 2 * 45000, holoenzyme, SDS-PAGE Columba livia
monomer 1 * 45000, apoenzyme, SDS-PAGE Gallus gallus
tetramer 4 * 50000, mitochondrial holoenzyme, antibody cross reaction Gallus gallus
tetramer 4 * 50000, mitochondrial holoenzyme, antibody cross reaction Columba livia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Gallus gallus
9
-
-
Columba livia

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Gallus gallus
pyridoxal 5'-phosphate
-
Columba livia