Activating Compound | Comment | Organism | Structure |
---|---|---|---|
aminooxyacetic acid | for some enzyme mutants, sensitivity to trypsin can be ameliorated by addition of pyridoxal 5'-phosphate or aminooxyacetic acid | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F52I | natural mutation in enzyme major allele, 13% of the activity of major allele | Homo sapiens |
F52I | natural mutation in enzyme minor allele, 14% of the activity of minor allele | Homo sapiens |
G41R | natural mutation in enzyme major allele, 46.5% of the activity of major allele | Homo sapiens |
G41R | natural mutation in enzyme minor allele, 23.7% of the activity of minor allele | Homo sapiens |
R233C | natural mutation in enzyme major allele, 21% of the activity of major allele | Homo sapiens |
R233C | natural mutation in enzyme minor allele, below 5% of the activity of minor allele | Homo sapiens |
V336D | natural mutation in enzyme major allele, 22.4% of the activity of major allele | Homo sapiens |
V336D | natural mutation in enzyme minor allele, 5.2% of the activity of minor allele | Homo sapiens |
General Stability | Organism |
---|---|
partial digestion by trypsin provides an indicator of proper folding of the enzyme, while for some mutants, sensitivity to trypsin can be ameliorated by addition of pyridoxal 5'-phosphate or aminooxyacetic acid | Homo sapiens |
partial trypsin digestion provides an indicator of proper folding of the mutant enzyme. For selected mutations the sensitivity to trypsin can be ameliorated by addition of pyridoxal phosphate or aminooxy acetic acid as specific pharmacological chaperones | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Homo sapiens | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, SDS-PAGE | Homo sapiens |
More | analysis of wild-type and mutants after partial digestions using trypsin. Partial digestion by trypsin provides an indicator of proper folding of the enzyme, while for some mutants, sensitivity to trypsin can be ameliorated by addition of pyridoxal 5'-phosphate or aminooxyacetic acid | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
alanine:glyoxylate aminotransferase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | for some mutants, sensitivity to trypsin can be ameliorated by addition of pyridoxal 5'-phosphate or aminooxyacetic acid | Homo sapiens |