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Literature summary for 2.6.1.44 extracted from
- Purification of three aminotransferases from Hydrogenobacter thermophilus TK-6 - novel types of alanine or glycine aminotransferase (2010), FEBS J., 277, 1876-1885.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
glyoxylate | in 50 mM NaPO4 (pH 8.0), at 70°C | Hydrogenobacter thermophilus |  |
| 8.1 | - |
L-alanine | in 50 mM NaPO4 (pH 8.0), at 70°C | Hydrogenobacter thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69000 | - |
gel filtration | Hydrogenobacter thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hydrogenobacter thermophilus | D2Z0I0 | - |
- |
| Hydrogenobacter thermophilus TK-6 / IAM 12695 | D2Z0I0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| butyl-Toyopearl column chromatography, DEAE-Toyopearl column chromatography, hydroxyapatite column chromatography, MonoQ column chromatography, and phenyl Superose gel filtration | Hydrogenobacter thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.071 | - |
enzyme from cell free extract | Hydrogenobacter thermophilus |
| 19 | - |
after 270fold purification | Hydrogenobacter thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-alanine + glyoxylate | high substrate specificity for Ala and glyoxylate | Hydrogenobacter thermophilus | pyruvate + glycine | - |
ir | |
| L-alanine + glyoxylate | high substrate specificity for Ala and glyoxylate | Hydrogenobacter thermophilus TK-6 / IAM 12695 | pyruvate + glycine | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | - |
Hydrogenobacter thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AGT | - |
Hydrogenobacter thermophilus |
| alanine:glyoxylate aminotransferase | - |
Hydrogenobacter thermophilus |
| aminotransferase 3 | - |
Hydrogenobacter thermophilus |
| AT3 | - |
Hydrogenobacter thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | 8.4 | - |
Hydrogenobacter thermophilus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Hydrogenobacter thermophilus | |
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Release 2023.1 (January 2023)
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