Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
I249A | reduced catalytic activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
67 | - |
L-tyrosine | mutant enzyme I249A | Homo sapiens | |
580 | - |
p-hydroxyphenylpyruvate | wild type enzyme | Homo sapiens | |
870 | - |
L-tyrosine | wild type enzyme | Homo sapiens | |
3300 | - |
2-oxoglutarate | wild type enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
NTA-resin column chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + 4-hydroxyphenylpyruvate | - |
Homo sapiens | 2-oxoglutarate + L-tyrosine | - |
? | |
L-tyrosine + 2-oxoglutarate | - |
Homo sapiens | 4-hydroxyphenylpyruvate + L-glutamate | - |
? | |
additional information | no detectable transamination of aspartate or other cosubstrates | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TATase | - |
Homo sapiens |
tyrosine aminotransferase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
2-oxoglutarate | wild type enzyme | Homo sapiens | |
0.3 | - |
2-oxoglutarate | mutant enzyme I249A | Homo sapiens | |
1 | - |
L-tyrosine | wild type enzyme | Homo sapiens | |
1.2 | - |
p-hydroxyphenylpyruvate | wild type enzyme | Homo sapiens | |
1.2 | - |
L-tyrosine | mutant enzyme I249A | Homo sapiens | |
3.8 | - |
L-glutamate | wild type enzyme | Homo sapiens | |
69 | - |
L-glutamate | wild type enzyme | Homo sapiens | |
83 | - |
L-tyrosine | wild type enzyme | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent | Homo sapiens |