Cloned (Comment) | Organism |
---|---|
recombinantly expressed in Escherichia coli as a His-tagged fusion protein | Variovorax paradoxus |
Crystallization (Comment) | Organism |
---|---|
the crystal structures of the holoenzyme and of the enzyme in complex with the inhibitor 2-aminooxyacetate reveal structural similarity to the beta-phenylalanine aminotransferase from Mesorhizobium sp. strain LUK | Variovorax paradoxus |
Protein Variants | Comment | Organism |
---|---|---|
R41A | mutant is inactive when tested with (S)-beta-phenylalanine as amino donor and 2-oxoglutarate as amino acceptor | Variovorax paradoxus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-aminooxyacetate | - |
Variovorax paradoxus | |
2-oxoglutarate | enzyme shows substrate inhibition | Variovorax paradoxus | |
beta-phenylalanine | enzyme shows substrate inhibition | Variovorax paradoxus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
2-oxoglutarate | pH 7.6, 30°C | Variovorax paradoxus | |
1.5 | - |
(S)-beta-phenylalanine | pH 7.6, 30°C | Variovorax paradoxus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46420 | - |
calculated from cDNA | Variovorax paradoxus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Variovorax paradoxus | H8WR05 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Variovorax paradoxus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-amino-5-methyl-hexanoic acid + 2-oxoglutarate | relative activity: 48% | Variovorax paradoxus | ? | - |
? | |
(S)-beta-phenylalanine + 2-oxoglutarate | relative activity: 100% | Variovorax paradoxus | 3-oxo-3-phenylpropionic acid + L-glutamate | - |
? | |
(S)-beta-phenylalanine + pyruvate | the activity of VpAT with pyruvate is 85% of that with alpha-ketoglutarate as the amino acceptor | Variovorax paradoxus | 3-oxo-3-phenylpropionic acid + L-alanine | - |
? | |
rac-2-fluoro-beta-phenylalanine + 2-oxoglutarate | relative activity: 15% | Variovorax paradoxus | ? | - |
? | |
rac-2-methyl-beta-phenylalanine + 2-oxoglutarate | relative activity: 5% | Variovorax paradoxus | ? | - |
? | |
rac-3-bromo-beta-phenylalanine + 2-oxoglutarate | relative activity: 53% | Variovorax paradoxus | ? | - |
? | |
rac-3-chloro-beta-phenylalanine + 2-oxoglutarate | relative activity: 56% | Variovorax paradoxus | ? | - |
? | |
rac-3-fluoro-beta-phenylalanine + 2-oxoglutarate | relative activity: 48% | Variovorax paradoxus | ? | - |
? | |
rac-3-methyl-beta-phenylalanine + 2-oxoglutarate | relative activity: 54% | Variovorax paradoxus | ? | - |
? | |
rac-4-bromo-beta-phenylalanine + 2-oxoglutarate | relative activity: 62% | Variovorax paradoxus | ? | - |
? | |
rac-4-ethyl-beta-phenylalanine + 2-oxoglutarate | relative activity: 134% | Variovorax paradoxus | ? | - |
? | |
rac-4-fluoro-beta-phenylalanine + 2-oxoglutarate | relative activity: 87% | Variovorax paradoxus | ? | - |
? | |
rac-4-iso-propyl-beta-phenylalanine + 2-oxoglutarate | relative activity: 61% | Variovorax paradoxus | ? | - |
? | |
rac-4-methyl-beta-phenylalanine + 2-oxoglutarate | relative activity: 100% | Variovorax paradoxus | ? | - |
? | |
rac-4-nitro-beta-phenylalanine + 2-oxoglutarate | relative activity: 32% | Variovorax paradoxus | ? | - |
? | |
rac-4-nitro-hydroxy-phenylalanine + 2-oxoglutarate | relative activity: 61% | Variovorax paradoxus | ? | - |
? | |
rac-4-propyl-beta-phenylalanine + 2-oxoglutarate | relative activity: 126% | Variovorax paradoxus | ? | - |
? | |
rac-4-trifluoromethyl-beta-phenylalanine + 2-oxoglutarate | relative activity: 73% | Variovorax paradoxus | ? | - |
? | |
rac-beta-leucine + 2-oxoglutarate | relative activity: 38% | Variovorax paradoxus | ? | - |
? | |
rac-beta-phenylalanine + 2-oxoglutarate | relative activity: 100% | Variovorax paradoxus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-phenylalanine aminotransferase | - |
Variovorax paradoxus |
VpAT | - |
Variovorax paradoxus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Variovorax paradoxus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.6 | - |
2-oxoglutarate | pH 7.6, 30°C | Variovorax paradoxus | |
11.8 | - |
(S)-beta-phenylalanine | pH 7.6, 30°C | Variovorax paradoxus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Variovorax paradoxus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 11.2 | enzyme shows high activity over a broad pH range | Variovorax paradoxus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Variovorax paradoxus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
40.3 | - |
beta-phenylalanine | enzyme shows substrate inhibition, pH 7.6, 30°C | Variovorax paradoxus | |
82.4 | - |
2-oxoglutarate | enzyme shows substrate inhibition, pH 7.6, 30°C | Variovorax paradoxus |