Cloned (Comment) | Organism |
---|---|
cDNA cloned and expressed in Escherichia coli DH1 | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0246 | - |
glyoxylate | pH 8.0, 30°C, native enzyme | Rattus norvegicus | |
0.0336 | - |
glyoxylate | pH 8.0, 30°C, recombinant enzyme | Rattus norvegicus | |
1.1 | - |
pyruvate | pH 8.0, 30°C, native and recombinant enzyme | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rattus norvegicus | 5737 | - |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
peroxisome | - |
Rattus norvegicus | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
2 * 43000, recombinant enzyme, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + pyruvate | Rattus norvegicus | - |
3-hydroxypyruvate + L-alanine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native and recombinant enzyme | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.41 | - |
recombinant enzyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + 3-hydroxypyruvate | - |
Rattus norvegicus | pyruvate + L-serine | - |
r | |
L-serine + pyruvate | - |
Rattus norvegicus | 3-hydroxypyruvate + L-alanine | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43000, recombinant enzyme, SDS-PAGE | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Rattus norvegicus |