General Stability | Organism |
---|---|
10% glycerol and 0.0001 mM pyridoxal 5'-phosphate stabilize | Azospirillum brasilense |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
indole-3-pyruvic acid | competitive inhibition | Azospirillum brasilense |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
L-tyrosine | - |
Azospirillum brasilense | |
0.35 | - |
L-histidine | - |
Azospirillum brasilense | |
0.36 | - |
L-5-fluorotryptophan | - |
Azospirillum brasilense | |
0.43 | - |
L-phenylalanine | - |
Azospirillum brasilense | |
0.58 | - |
2-oxoglutarate | cosubstrate L-tyrosine | Azospirillum brasilense | |
1.05 | - |
L-tryptophan | - |
Azospirillum brasilense | |
1.2 | - |
L-5-methyltryptophan | - |
Azospirillum brasilense | |
1.44 | - |
2-oxoglutarate | cosubstrate L-tryptophan | Azospirillum brasilense |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
2 * 33000, SDS-PAGE | Azospirillum brasilense |
66000 | - |
gel filtration | Azospirillum brasilense |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azospirillum brasilense | - |
rhizosphere microorganism, strain UAP 14, aromatic amino acid transaminase AAT1, predominant isoform | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-Sephacel, hydroxylapatite, Sephadex G-100, phenyl-Sepharose | Azospirillum brasilense |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26 | - |
- |
Azospirillum brasilense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-5-fluorotryptophan + 2-oxoglutarate | - |
Azospirillum brasilense | 3-indole-2-oxopropanoate + L-glutamate | - |
? | |
L-5-methyltryptophan + 2-oxoglutarate | - |
Azospirillum brasilense | 3-indole-2-oxopropanoate + L-glutamate | - |
? | |
L-histidine + 2-oxoglutarate | 28% of activity with L-tyrosine | Azospirillum brasilense | 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-glutamate | - |
? | |
L-phenylalanine + 2-oxoglutarate | very low activity with L-aspartate, L-valine and L-isoleucine | Azospirillum brasilense | phenylpyruvate + L-glutamate | - |
? | |
L-tryptophan + 2-oxoglutarate | - |
Azospirillum brasilense | 3-indole-2-oxopropanoate + L-glutamate | - |
? | |
L-tyrosine + 2-oxoglutarate | best amino donor | Azospirillum brasilense | p-hydroxyphenylpyruvate + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 33000, SDS-PAGE | Azospirillum brasilense |
Synonyms | Comment | Organism |
---|---|---|
AAT1 | - |
Azospirillum brasilense |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
with L-phenylalanine and L-tyrosine as substrates | Azospirillum brasilense |
8.5 | - |
with L-tryptophan and its derivatives as substrates | Azospirillum brasilense |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | 50% of maximal activity at pH 6.0 and pH 9.5 | Azospirillum brasilense |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
indole-3-pyruvic acid | - |
Azospirillum brasilense |