Application | Comment | Organism |
---|---|---|
synthesis | asymmetric synthesis of L-homophenylalanine and L-phenylglycine from 2-oxo-4-phenylbutyrate and phenylglyoxylate, respectively, using L-glutamate as an amino donor. AroATBs can be used as a biocatalyst for the synthesis of unnatural L-amino acids by taking advantage of the large solubility difference between substrate and product at higher temperatures to accelerate an equilibrium shift favoring product formation | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Bacillus sp. (in: Bacteria) T30 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + 2-oxo-4-phenylbutyrate | - |
Bacillus sp. (in: Bacteria) | L-homophenylalanine + 2-oxoglutarate | - |
r | |
L-glutamate + 2-oxo-4-phenylbutyrate | - |
Bacillus sp. (in: Bacteria) T30 | L-homophenylalanine + 2-oxoglutarate | - |
r | |
L-glutamate + phenylglyoxylate | - |
Bacillus sp. (in: Bacteria) | L-phenylglycine + 2-oxoglutarate | - |
r | |
L-glutamate + phenylglyoxylate | - |
Bacillus sp. (in: Bacteria) T30 | L-phenylglycine + 2-oxoglutarate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 80 | 37°C: about 35% of maximal activity, 50°C: about 90% of maximal activity, 80°C: about 50% of maximal activity | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
half-life: 28 h | Bacillus sp. (in: Bacteria) |
60 | - |
half-life: 12 h | Bacillus sp. (in: Bacteria) |
70 | - |
half-life: 4.2 h | Bacillus sp. (in: Bacteria) |
80 | - |
half-life: 1.1 h | Bacillus sp. (in: Bacteria) |