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Literature summary for 2.6.1.57 extracted from

  • Hirata, H.; Ohnishi, T.; Ishida, H.; Tomida, K.; Sakai, M.; Hara, M.; Watanabe, N.
    Functional characterization of aromatic amino acid aminotransferase involved in 2-phenylethanol biosynthesis in isolated rose petal protoplasts (2012), J. Plant Physiol., 169, 444-451.
    View publication on PubMed
Search for more literature for 2.6.1.57

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rosa hybrid cultivar

Inhibitors

Inhibitors Comment Organism Structure
carbidopa inhibitor of pyridoxal phosphate-dependent enzymes. almost complete inhibition at 0.5 mM Rosa hybrid cultivar

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46300
-
 x * 46300, calculated Rosa hybrid cultivar

Organism

Organism UniProt Comment Textmining
Rosa hybrid cultivar H7CE71
-
-

Source Tissue

Source Tissue Comment Organism Textmining
calyx
-
 Rosa hybrid cultivar
-
flower
-
 Rosa hybrid cultivar
-
fruit
-
 Rosa hybrid cultivar
-
leaf
-
 Rosa hybrid cultivar
-
petal
-
 Rosa hybrid cultivar
-
stem
-
 Rosa hybrid cultivar
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + oxaloacetate
-
 Rosa hybrid cultivar phenylpyruvate + ?
-
 ?
L-tryptophan + 2-oxoglutarate at about 25% of the rate with L-phenylalanine Rosa hybrid cultivar 3-indole-2-oxopropanoate + L-glutamate
-
 ?
L-tyrosine + 2-oxoglutarate at about 25% of the rate with L-phenylalanine Rosa hybrid cultivar 4-hydroxyphenylpyruvate + L-glutamate
-
 ?
additional information 2-oxoglutaric acid is the preferred amino acceptor rather than oxaloacetic acid. Enzyme shows the highest activity toward L-phenylalanine in comparison with L-tryptophan, L-tyrosine, D-phenylalanine, glycine, and L-alanine, and shows 9.7fold higher activity with L-phenylalanine rather than phenylpyruvate as a substrate Rosa hybrid cultivar ?
-
 ?

Subunits

Subunits Comment Organism
? x * 46300, calculated Rosa hybrid cultivar

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Rosa hybrid cultivar

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Rosa hybrid cultivar

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate sequence includes a aminotransferases family-I pyridoxal phosphate attachment site, SLSKrwLVpGWRLG Rosa hybrid cultivar

pI Value

Organism Comment pI Value Maximum pI Value
Rosa hybrid cultivar
-
-
 6.3

General Information

General Information Comment Organism
physiological function RNAi suppression of the enzyme decreases 2-phenylethanol production Rosa hybrid cultivar